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On-tissue protein identification and imaging by MALDI-Ion mobility mass spectrometry

  • Jonathan Stauber
  • Luke MacAleese
  • Julien Franck
  • Emmanuelle Claude
  • Marten Snel
  • Basak Kükrer Kaletas
  • Ingrid M. V. D. Wiel
  • Maxence Wisztorski
  • Isabelle Fournier
  • Ron M. A. HeerenEmail author
Article

Abstract

MALDI imaging mass spectrometry (MALDI-IMS) has become a powerful tool for the detection and localization of drugs, proteins, and lipids on-tissue. Nevertheless, this approach can only perform identification of low mass molecules as lipids, pharmaceuticals, and peptides. In this article, a combination of approaches for the detection and imaging of proteins and their identification directly on-tissue is described after tryptic digestion. Enzymatic digestion protocols for different kinds of tissues—formalin fixed paraffin embedded (FFPE) and frozen tissues—are combined with MALDI-ion mobility mass spectrometry (IM-MS). This combination enables localization and identification of proteins via their related digested peptides. In a number of cases, ion mobility separates isobaric ions that cannot be identified by conventional MALDI time-of-flight (TOF) mass spectrometry. The amount of detected peaks per measurement increases (versus conventional MALDI-TOF), which enables mass and time selected ion images and the identification of separated ions. These experiments demonstrate the feasibility of direct proteins identification by ion-mobility-TOF IMS from tissue. The tissue digestion combined with MALDI-IM-TOF-IMS approach allows a proteomics “bottom-up” strategy with different kinds of tissue samples, especially FFPE tissues conserved for a long time in hospital sample banks. The combination of IM with IMS marks the development of IMS approaches as real proteomic tools, which brings new perspectives to biological studies.

Keywords

MALDI Drift Time Image Mass Spectrometry MALDI Image Mass Spectrometric Image 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  • Jonathan Stauber
    • 1
  • Luke MacAleese
    • 1
    • 4
  • Julien Franck
    • 2
  • Emmanuelle Claude
    • 3
  • Marten Snel
    • 3
  • Basak Kükrer Kaletas
    • 1
  • Ingrid M. V. D. Wiel
    • 1
  • Maxence Wisztorski
    • 2
  • Isabelle Fournier
    • 2
  • Ron M. A. Heeren
    • 1
    • 4
    Email author
  1. 1.Macromolecular Ion Physics, Biomolecular Mass SpectrometryFOM Institute for Atomic and Molecular PhysicsAmsterdamThe Netherlands
  2. 2.MALDI Imaging TeamUniversity of LilleLilleFrance
  3. 3.Waters CorporationManchesterUnited Kingdom
  4. 4.The Netherlands Proteomics CentreUtrechtThe Netherlands

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