MALDI produced ions inspected with a linear ion trap-orbitrap hybrid mass analyzer

  • Kerstin Strupat
  • Viatcheslav Kovtoun
  • Huy Bui
  • Rosa Viner
  • George Stafford
  • Stevan Horning
Focus: The Orbitrap


A MALDI source is interfaced to a modified LTQ Orbitrap XL instrument. This work gives insight into the MALDI source design and shows results obtained with the MALDI source coupled to an accurate mass, high-resolution hybrid mass spectrometer. MALDI-produced ions and fragment ions thereof produced in the mass spectrometer may be analyzed and detected by the Orbitrap analyzer at a maximum mass resolution of 100,000 (FWHM) at m/z 400 with high mass accuracy. An accuracy of ≤2 ppm is achieved by internal mass calibration using lock mass functionality; using external mass calibration, an accuracy of ≤3 ppm is routinely obtained. External mass calibration of the hybrid mass spectrometer is performed using a standard calibration mixture of different peptides and matrix components. The instrumental capabilities are demonstrated for analytical methodologies such as Protein ID using Peptide Mass Fingerprint (PMF) and MS/MS analyses of small molecule samples. Stability of mass accuracy and signal-to-noise ratio for low samples loads (on plates) are demonstrated as well as the experimental dynamic range using α-cyano-4-hydroxy cinnamic acid (CHCA) matrix.


MALDI Laser Shot Automatic Gain Control Linear Trap CHCA Matrix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Supplementary material

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Copyright information

© American Society for Mass Spectrometry 2009

Authors and Affiliations

  • Kerstin Strupat
    • 1
  • Viatcheslav Kovtoun
    • 2
  • Huy Bui
    • 2
  • Rosa Viner
    • 2
  • George Stafford
    • 2
  • Stevan Horning
    • 1
  1. 1.Thermo Fisher Scientific (Bremen) GmbHBremenGermany
  2. 2.Thermo Fisher Scientific San JoseSan JoseUSA

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