High-throughput quantitative analysis by desorption electrospray ionization mass spectrometry
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A newly developed high-throughput desorption electrospray ionization (DESI) source was characterized in terms of its performance in quantitative analysis. A 96-sample array, containing pharmaceuticals in various matrices, was analyzed in a single run with a total analysis time of 3 min. These solution-phase samples were examined from a hydrophobic PTFE ink printed on glass. The quantitative accuracy, precision, and limit of detection (LOD) were characterized. Chemical background-free samples of propranolol (PRN) with PRN-d7 as internal standard (IS) and carbamazepine (CBZ) with CBZ-d10 as IS were examined. So were two other sample sets consisting of PRN/PRN-d7 at varying concentration in a biological milieu of 10% urine or porcine brain total lipid extract, total lipid concentration 250 ng/µL. The background-free samples, examined in a total analysis time of 1. 5 s/sample, showed good quantitative accuracy and precision, with a relative error (RE) and relative standard deviation (RSD) generally less than 3% and 5%, respectively. The samples in urine and the lipid extract required a longer analysis time (2. 5 s/sample) and showed RSD values of around 10% for the samples in urine and 4% for the lipid extract samples and RE values of less than 3% for both sets. The LOD for PRN and CBZ when analyzed without chemical background was 10 and 30 fmol, respectively. The LOD of PRN increased to 400 fmol analyzed in 10% urine, and 200 fmol when analyzed in the brain lipid extract.
KeywordsImage Mass Spectrometry Peak Height Ratio Total Analysis Time Desorption Electrospray Ionization Biological Milieu
- 3.Kovarik, P.; Grivet, C.; Bourgogne, E.; Hopfgartner, G. Method Development Aspects for the Quantitation of Pharmaceutical Compounds in Human Plasma with a Matrix-Assisted Laser Desorption/Ionization Source in the Multiple Reaction Monitoring Mode. Rapid Commun. Mass Spectrom. 2007, 21, 911–919.CrossRefGoogle Scholar
- 6.Sampson, J. S.; Hawkridge, A. M.; Muddiman, D. C. Generation and Detection of Multiply-Charged Peptides and Proteins by Matrix-Assisted Laser Desorption Electrospray Ionization (MALDESI) Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2006, 17(12), 1712–1716.CrossRefGoogle Scholar
- 14.Williams, J. P.; Hilton, G. R.; Thalassinos, K.; Jackson, A. T.; Scrivens, J. H. The Rapid Characterization of Poly(Ethylene Glycol) Oligomers Using Desorption Electrospray Ionization Tandem Mass Spectrometry Combined with Novel Product Ion Peak Assignment Software. Rapid Commun. Mass Spectrom. 2007, 21(11), 1693–1704.CrossRefGoogle Scholar
- 20.Manicke, N. E.; Wiseman, J. M.; Ifa, D. R.; Cooks, R. G. Desorption Electrospray Ionization (DESI) Mass Spectrometry and Tandem Mass Spectrometry (MS/MS) of Phospholipids and Sphingolipids: Ionization, Adduct Formation, and Fragmentation. J. Am. Soc. Mass Spectrom. 2008, 19(4), 531–543.CrossRefGoogle Scholar