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Alternative reagents for chemical noise reduction in liquid chromatography-mass spectrometry using selective ion-molecule reactions

  • Xinghua Guo
  • Andries P. Bruins
  • Thomas R. Covey
  • Martin Trötzmüller
  • Ernst Lankmayr
Articles

Abstract

Reduction of ionic chemical background noise based on selective gas-phase reactions with chosen neutral reagents has been proven to be a very promising approach in liquid chromatography—mass spectrometry (LC-MS). In this study further investigations on alternative reagents including the disulfides (dimethyl disulfide, diethyl disulfide, methyl propyl disulfide), dimethyl trisulfide, ethylene oxide, and butadiene monoxide, for example, have been carried out. Tandem mass spectrometric studies of ion/molecule reactions indicate that—besides dimethyl disulfide—ethylene oxide and butadiene monoxide also exhibit very efficient reactions with background ions. Furthermore, it is confirmed that the reactions are very selective according to the test with some analyte ions. In contrast to its rapid reactions with background ions, ethylene oxide does not react, or reacts much less, with these analytes. Therefore, it can be used as an alternative reagent for noise reduction. Although reactions of the other tested neutral reagents with background ions are evaluated, they are generally not suitable as reagents for this purpose because of lack of reactivity or dramatic ion losses during reactions.

Keywords

Ethylene Oxide Ethylene Oxide DMDS Dimethyl Disulfide Trisulfide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Society for Mass Spectrometry 2009

Authors and Affiliations

  • Xinghua Guo
    • 1
  • Andries P. Bruins
    • 2
  • Thomas R. Covey
    • 3
  • Martin Trötzmüller
    • 1
  • Ernst Lankmayr
    • 1
  1. 1.Institute of Analytical Chemistry and RadiochemistryGraz University of TechnologyGraz, SteiermarkAustria
  2. 2.Mass Spectrometry Core FacilityUniversity of GroningenGroningenThe Netherlands
  3. 3.Applied Biosystems/MDS SciexConcordCanada

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