Detection of honeybee venom in envenomed tissues by direct MALDI MSI

  • Simona Francese
  • Duccio Lambardi
  • Guido Mastrobuoni
  • Giancarlo la Marca
  • Gloriano Moneti
  • Stefano Turillazzi
Articles

Abstract

A new analytical approach using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) for the study of honeybee venom is shown. In vitro and in vivo models simulating the bee sting have been developed using live honeybees and, as the envenomation sites, pig ears and rat legs; MALDI MSI has been used to map, over time, the diffusion and distribution of three venom allergens (Api m 1, Api m 4, and Api m 6) and two venom toxins (apamine and mast cell degranulating peptide). In conjunction with other classical biochemical techniques and high resolution mass spectrometry (HRMS), structural data have been obtained that contribute to current understanding of honeybee venom composition. Initial data have also been obtained demonstrating the feasibility of mapping the organism’s response to the sting. The opportunity to monitor venom diffusion and the organism’s response at the same time might open new pathways for in vivo preclinical studies in designing and testing new venom immunotherapy (VIT).

Supplementary material

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Copyright information

© American Society for Mass Spectrometry 2009

Authors and Affiliations

  • Simona Francese
    • 1
  • Duccio Lambardi
    • 2
  • Guido Mastrobuoni
    • 1
  • Giancarlo la Marca
    • 3
  • Gloriano Moneti
    • 1
  • Stefano Turillazzi
    • 2
  1. 1.Interdepartmental Centre of Mass SpectrometryUniversity of FlorenceFlorenceItaly
  2. 2.Department of Evolutionary BiologyUniversity of FlorenceFlorenceItaly
  3. 3.Department of PharmacologyUniversity of Florence, Mass Spectrometry Laboratory, Metabolic and Neuromuscular Unit, Meyer Children’s HospitalFlorenceItaly
  4. 4.Department of Health and Wellbeing, Biomedical Research Center (BMRC)Sheffield Hallam UniversitySheffieldUK

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