A linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions

  • Matthew W. Soyk
  • Qin Zhao
  • R. S. Houk
  • Ethan R. Badman
Articles

Abstract

A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument’s complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.

References

  1. 1.
    Douglas, D. J.; Frank, A. J.; Mao, D. M. Linear ion traps in mass spectrometry. Mass Spectrom. Rev. 2005, 24, 1–29.CrossRefGoogle Scholar
  2. 2.
    Campbell, J. M.; Collings, B. A.; Douglas, D. J. A new linear ion trap time-of-flight system with tandem mass spectrometry capabilities. Rapid Commun. Mass Spectrom. 1998, 12, 1463–1474.CrossRefGoogle Scholar
  3. 3.
    Collings, B. A.; Campbell, J. M.; Mao, D. M.; Douglas, D. J. A combined linear ion trap time-of-flight system with improved performance and MSn capabilities. Rapid Commun. Mass Spectrom. 2001, 15, 1777–1795.CrossRefGoogle Scholar
  4. 4.
    Harkewicz, R.; Belov, M. E.; Anderson, G. A.; Pasa-Tolic, L.; Masselon, C. D.; Prior, D. C.; Udseth, H. R.; Smith, R. D. ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation. J. Am. Soc. Mass Spectrom. 2002, 13, 144–154.CrossRefGoogle Scholar
  5. 5.
    Syka, J. E. P.; Marto, J. A.; Bai, D. L.; Horning, S.; Senko, M. W.; Schwartz, J. C.; Ueberheide, B.; Garcia, B.; Busby, S.; Muratore, T.; Shabanowitz, J.; Hunt, D. F. Novel linear quadrupole ion trap/FT mass spectrometer: Performance characterization and use in the comparative analysis of histone H3 post-translational modifications. J. Proteome Res. 2004, 3, 621–626.CrossRefGoogle Scholar
  6. 6.
    Schwartz, J. C.; Senko, M. W.; Syka, J. E. P. A two-dimensional quadrupole ion trap mass spectrometer. J. Am. Soc. Mass Spectrom. 2002, 13, 659–669.CrossRefGoogle Scholar
  7. 7.
    Hager, J. W. A new linear ion trap mass spectrometer. Rapid Commun. Mass Spectrom. 2002, 16, 512–526.CrossRefGoogle Scholar
  8. 8.
    Jonscher, K. R.; Yates, J. R. The quadrupole ion trap mass spectrometer—a small solution to a big challenge. Anal. Biochem. 1997, 244, 1–15.CrossRefGoogle Scholar
  9. 9.
    Reid, G. E.; McLuckey, S. A. ‘Top down’ protein characterization via tandem mass spectrometry. J. Mass Spectrom. 2002, 37, 663–675.CrossRefGoogle Scholar
  10. 10.
    Hu, Q. Z.; Noll, R. J.; Li, H. Y.; Makarov, A.; Hardman, M.; Cooks, R. G. The Orbitrap: a new mass spectrometer. J. Mass Spectrom. 2005, 40, 430–443.CrossRefGoogle Scholar
  11. 11.
    Syka, J. E. P.; Coon, J. J.; Schroeder, M. J.; Shabanowitz, J.; Hunt, D. F. Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 9528–9533.CrossRefGoogle Scholar
  12. 12.
    Xia, Y.; Wu, J.; McLuckey, S. A.; Londry, F. A.; Hager, J. W. Mutual storage mode ion/ion reactions in a hybrid linear ion trap. J. Am. Soc. Mass Spectrom. 2005, 16, 71–81.CrossRefGoogle Scholar
  13. 13.
    Stephenson, J. L.; McLuckey, S. A. Simplification of product ion spectra derived from multiply charged parent ions via ion/ion chemistry. Anal. Chem. 1998, 70, 3533–3544.CrossRefGoogle Scholar
  14. 14.
    Londry, F. A.; Hager, J. W. Mass selective axial ion ejection from a linear quadrupole ion trap. J. Am. Soc. Mass Spectrom. 2003, 14, 1130–1147.CrossRefGoogle Scholar
  15. 15.
    Liang, X. R.; McLuckey, S. A. Transmission mode ion/ion proton transfer reactions in a linear ion trap. J. Am. Soc. Mass Spectrom. 2007, 18, 882–890.CrossRefGoogle Scholar
  16. 16.
    Liang, X. R.; Han, H. L.; Xia, Y.; McLuckey, S. A. A pulsed triple ionization source for sequential ion/ion reactions in an electrodynamic ion trap. J. Am. Soc. Mass Spectrom. 2007, 18, 369–376.CrossRefGoogle Scholar
  17. 17.
    Xia, Y.; Liang, X. R.; McLuckey, S. A. Pulsed dual electrospray ionization for ion/ion reactions. J. Am. Soc. Mass Spectrom. 2005, 16, 1750–1756.CrossRefGoogle Scholar
  18. 18.
    Xia, Y.; Chrisman, P. A.; Erickson, D. E.; Liu, J.; Liang, X. R.; Londry, F. A.; Yang, M. J.; McLuckey, S. A. Implementation of ion/ion reactions in a quadrupole/time-of-flight tandem mass spectrometer. Anal. Chem. 2006, 78, 4146–4154.CrossRefGoogle Scholar
  19. 19.
    Liang, X. R.; Hager, J. W.; McLuckey, S. A. Transmission mode ion/ion electron-transfer dissociation in a linear ion trap. Anal. Chem. 2007, 79, 3363–3370.CrossRefGoogle Scholar
  20. 20.
    Liang, X. R.; Xia, Y.; McLuckey, S. A. Alternately pulsed nanoelectrospray ionization/atmospheric pressure chemical ionization for ion/ion reactions in an electrodynamic ion trap. Anal. Chem. 2006, 78, 3208–3212.CrossRefGoogle Scholar
  21. 21.
    Han, H. L.; Xia, Y.; McLuckey, S. A. Ion trap collisional activation of c and z(center dot) ions formed via gas-phase ion/ion electron-transfer dissociation. J. Proteome. Res. 2007, 6, 3062–3069.CrossRefGoogle Scholar
  22. 22.
    Wu, J.; Hager, J. W.; Xia, Y.; Londry, F. A.; McLuckey, S. A. Positive ion transmission mode ion/ion reactions in a hybrid linear ion trap. Anal. Chem. 2004, 76, 5006–5015.CrossRefGoogle Scholar
  23. 23.
    Wells, J. M.; Chrisman, P. A.; McLuckey, S. A. “Dueling” ESI: Instrumentation to study ion/ion reactions of electrospray-generated cations and anions. J. Am. Soc. Mass Spectrom. 2002, 13, 614–622.CrossRefGoogle Scholar
  24. 24.
    Badman, E. R.; Chrisman, P. A.; McLuckey, S. A. A quadrupole ion trap mass spectrometer with three independent ion sources for the study of gas-phase ion/ion reactions. Anal. Chem. 2002, 74, 6237–6243.CrossRefGoogle Scholar
  25. 25.
    Zhao Q. Soyk M. W. Schieffer G. M. Houk R. S. Badman E. R. Fuhrer K. Gonin M. An ion trap-ion mobility-time of flight mass spectrometer with three ion sources for ion/ion reactions. J. Am. Soc. Mass Spectrom. 2008, unpublished.Google Scholar
  26. 26.
    Paul, W.; Reinhard, H. P.; Vonzahn, U. The electric mass filter as mass spectrometer and isotope separator. Zeitschrift fur Physik 1958, 152, 143–182.CrossRefGoogle Scholar
  27. 27.
    McLuckey, S. A.; Reid, G. E.; Wells, J. M. Ion parking during ion/ion reactions in electrodynamic ion traps. Anal. Chem. 2002, 74, 336–346.CrossRefGoogle Scholar
  28. 28.
    Chrisman, P. A.; Pitteri, S. J.; McLuckey, S. A. Parallel ion parking: Improving conversion of parents to first-generation products in electron transfer dissociation. Anal. Chem. 2005, 77, 3411–3414.CrossRefGoogle Scholar
  29. 29.
    Chrisman, P. A.; Pitteri, S. J.; McLuckey, S. A. Parallel ion parking of protein mixtures. Anal. Chem. 2006, 78, 310–316.CrossRefGoogle Scholar
  30. 30.
    Moradian, A.; Douglas, D. J. Mass selective axial ion ejection from linear quadrupoles with added octopole fields. J. Am. Soc. Mass Spectrom. 2008, 19, 270–280.CrossRefGoogle Scholar
  31. 31.
    Stephenson, J. L.; McLuckey, S. A. Adaptation of the Paul Trap for study of the reaction of multiply charged cations with singly charged anions. Int. J. Mass Spectrom. Ion Processes 1997, 162, 89–106.CrossRefGoogle Scholar
  32. 32.
    Mo, W.; Langford, M. L.; Todd, J. F. J. Investigation of ‘ghost’ peaks caused by nonlinear fields in the ion trap mass spectrometer. Rapid Commun. Mass Spectrom. 1995, 9, 107–113.CrossRefGoogle Scholar
  33. 33.
    Franzen, J.; Gabling, R. H.; Schubert, M.; Wang, Y. In Practical Aspects of Ion Trap Mass Spectrometry, Vol. I; March, R. E.; Todd, J. F. J., Eds.; CRC Press: Boca Raton, FL, 1995; Vol. 1, p. 49–167.Google Scholar

Copyright information

© American Society for Mass Spectrometry 2008

Authors and Affiliations

  • Matthew W. Soyk
    • 1
  • Qin Zhao
    • 1
  • R. S. Houk
    • 1
    • 2
  • Ethan R. Badman
    • 3
  1. 1.Department of ChemistryIowa State UniversityAmesUSA
  2. 2.Ames Laboratory, U.S. Department of EnergyIowa State UniversityAmesUSA
  3. 3.Nonclinical SafetyHoffman-La Roche Inc.NutleyUSA

Personalised recommendations