Journal of the American Society for Mass Spectrometry

, Volume 19, Issue 12, pp 1821–1831

A linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions

  • Matthew W. Soyk
  • Qin Zhao
  • R. S. Houk
  • Ethan R. Badman
Articles

Abstract

A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument’s complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.

Copyright information

© American Society for Mass Spectrometry 2008

Authors and Affiliations

  • Matthew W. Soyk
    • 1
  • Qin Zhao
    • 1
  • R. S. Houk
    • 1
    • 2
  • Ethan R. Badman
    • 3
  1. 1.Department of ChemistryIowa State UniversityAmesUSA
  2. 2.Ames Laboratory, U.S. Department of EnergyIowa State UniversityAmesUSA
  3. 3.Nonclinical SafetyHoffman-La Roche Inc.NutleyUSA
  4. 4.LECO CorporationSt. JosephUSA

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