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Surface-induced dissociation shows potential to be more informative than collision-induced dissociation for structural studies of large systems

  • Vicki H. Wysocki
  • Christopher M. Jones
  • Asiri S. Galhena
  • Anne E. Blackwell
Critical Insight
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Revised:
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Abstract

The ability to preserve noncovalent, macromolecular assemblies intact in the gas phase has paved the way for mass spectrometry to characterize ions of increasing size and become a powerful tool in the field of structural biology. Tandem mass spectrometry experiments have the potential to expand the capabilities of this technique through the gas-phase dissociation of macromolecular complexes, but collisions with small gas atoms currently provide very limited fragmentation. One alternative for dissociating large ions is to collide them into a surface, a more massive target. Here, we demonstrate the ability and benefit of fragmenting large protein complexes and inorganic salt clusters by surface-induced dissociation (SID), which provides more extensive fragmentation of these systems and shows promise as an activation method for ions of increasing size.

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Copyright information

© American Society for Mass Spectrometry 2008

Authors and Affiliations

  • Vicki H. Wysocki
    • 1
  • Christopher M. Jones
    • 1
  • Asiri S. Galhena
    • 1
  • Anne E. Blackwell
    • 1
  1. 1.Department of Chemistry, Department of Biochemistry and Molecular BiophysicsUniversity of ArizonaTucsonUSA

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