Advertisement

Imaging of a β-peptide distribution in whole-body mice sections by MALDI mass spectrometry

  • Markus StoeckliEmail author
  • Dieter Staab
  • Alain Schweitzer
  • James Gardiner
  • Dieter Seebach
Short Communication

Abstract

Mass spectrometric imaging was applied to assess compound distributions on whole-body sections of mice after i.v. dosing of a β-peptide and an α-peptide control. Animals were sacrificed at different time points (5 min, 1 h, 24 h) post-dose, providing simultaneous spatial as well as kinetic information. As a result of this study, no detection of the α-peptide control was observed at 1 h post-dose, while retention of the β-peptide was observed for longer than 24 h post-dose.

Keywords

Peptide MALDI MALDI Mass Spectrometry Kidney Region Mass Spectrometric Imaging 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Caprioli, R. M.; Farmer, T. B.; Gile, J. Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS. Anal. Chem. 1997, 69, 4751–4760.CrossRefGoogle Scholar
  2. 2.
    Pierson, J.; Norris, J. L.; Aerni, H. R.; Svenningsson, P.; Caprioli, R. M.; Andren, P. E. Molecular Profiling of Experimental Parkinson’s Disease: Direct Analysis of Peptides and Proteins on Brain Tissue Sections by MALDI Mass Spectrometry. J. Proteome Res. 2004, 3, 289–295.CrossRefGoogle Scholar
  3. 3.
    Caprioli, R. M.; Farmer, T. B.; Zhang, H.; Stoeckli, M. Molecular imaging of biological samples by MALDI MS. Proceedings of the 214th ACS National Meeting, Book of Abstracts; Las Vegas, NV, September, 1997; p ANYL-113.Google Scholar
  4. 4.
    Stoeckli, M.; Staab, D.; Staufenbiel, M.; Wiederhold, K. H.; Signor, L. Molecular Imaging of Amyloid β Peptides in Mouse Brain Sections Using Mass Spectrometry. Anal. Biochem. 2002, 311, 33–39.CrossRefGoogle Scholar
  5. 5.
    Stoeckli, M.; Staab, D.; Schweitzer, A. Compound and Metabolite Distribution Measured by MALDI Mass Spectrometric Imaging in Whole-Body Tissue Sections. Int. J. Mass Spectrom. 2007, 260, 195–202.CrossRefGoogle Scholar
  6. 6.
    Khatib-Shahidi, S.; Andersson, M.; Herman, J. L.; Gillespie, T. A.; Caprioli, R. M. Direct Molecular Analysis of Whole-Body Animal Tissue Sections by Imaging MALDI Mass Spectrometry. Anal. Chem. 2006, 78, 6448–6456.CrossRefGoogle Scholar
  7. 7.
    Seebach, D.; Beck, A. K.; Bierbaum, D. J. The World of β-and γ-Peptides Comprised of Homologated Proteinogenic Amino Acids and Other Components. Chem. Biodiversity. 2004, 1, 1111–1239.CrossRefGoogle Scholar
  8. 8.
    Nunn, C.; Rueping, M.; Langenegger, D.; Schuepbach, E.; Kimmerlin, T.; Micuch, P.; Hurth, K.; Seebach, D.; Hoyer, D. β(2)/β(3)-Di- and α/β(3)-Tetrapeptide Derivatives as Potent Agonists at Somatostatin SST(4) Receptors. Naunyn-Schmiedebergs Arch Pharmacol. 2003, 367, 95–103.CrossRefGoogle Scholar
  9. 9.
    Frackenpohl, J.; Arvidsson, P. I.; Schreiber, J. V.; Seebach, D. The Outstanding Biological Stability of β- and γ-Peptides Toward Proteolytic Enzymes: An in Vitro Investigation with Fifteen Peptidases. Chem. Biochem. 2001, 2, 445–455.Google Scholar
  10. 10.
    Weiss, H. M.; Wirz, B.; Schweitzer, A.; Amstutz, R.; Rodriguez-Perez, M. I.; Andres, H.; Metz, Y.; Gardiner, J.; Seebach, D. ADME-Investigations of Unnatural Peptides: Distribution of a 14C-labeled β-3-Octa-Arginine in Rats. Chem. Biodivers. 2007, 4, 1413–1437.CrossRefGoogle Scholar
  11. 11.
    Schreiber, J. V.; Quadroni, M.; Seebach, D. Sequencing of β-Peptides by Mass Spectrometry. Chimia. 1999, 53, 621–626.Google Scholar
  12. 12.
    Hook, D. F.; Bindschadler, P.; Mahajan, Y. R.; Sebesta, R.; Kast, P.; Seebach, D. The Proteolytic Stability of “Designed” β-Peptides Containing α-peptide-bond mimics and of mixed α,β-peptides: Application to the Construction of MHC-Binding Peptides. Chem. Biodiversity. 2005, 2, 591–632.CrossRefGoogle Scholar
  13. 13.
    Ullberg, S. The Technique of Whole-Body Autoradiography: Cryosectioning of Large Specimens. In Science Tools; Alvfeldt O., Ed.; Science Tools LKB-Producter AB: Stockholm, Sweden, 1977.Google Scholar

Copyright information

© American Society for Mass Spectrometry 2007

Authors and Affiliations

  • Markus Stoeckli
    • 1
    Email author
  • Dieter Staab
    • 1
  • Alain Schweitzer
    • 2
  • James Gardiner
    • 3
  • Dieter Seebach
    • 3
  1. 1.Novartis Institutes for BioMedical ResearchBaselSwitzerland
  2. 2.Exploratory Development, Drug Metabolism, and PharmacokineticsNovartisSwitzerland
  3. 3.Laboratory for Organic Chemistry, Department of Chemistry and BiosciencesETH-ZürichZürichSwitzerland

Personalised recommendations