Strategies for the analysis of poly(methacrylic acid) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

  • Mark F. Wyatt
  • Nicolas Schaeffer
  • Bien Tan
  • Andrew I. Cooper
Application Note


We evaluated several aqueous-based sample preparation protocols for the analysis of poly(methacrylic acid) (PMAA) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The sample contained a pentaerythritol tetra(3-mercaptopropionate) end-group, and was characterized in positive and negative ion modes using 2,5-dihydroxybenzoic acid (DHB) and 2,4,6-trihydroxyacetophenone (THAP) matrices. The major series observed were the [M + Na]+ species, in positive ion mode, and the [M − H] species, in negative ion mode. The performance of DHB and THAP matrices was comparable in positive ion mode, but THAP outperformed DHB in negative ion mode. The use of ion-exchange beads (IXB) benefited the analysis, while the addition of sodium acetate (NaOAc) or trifluoroacetic acid (TFA) proved disadvantageous in positive ion mode.


Atom Transfer Radical Polymerization Methacrylic Acid PMAA NaOAc Major Series 
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Copyright information

© American Society for Mass Spectrometry 2007

Authors and Affiliations

  • Mark F. Wyatt
    • 1
  • Nicolas Schaeffer
    • 2
  • Bien Tan
    • 2
  • Andrew I. Cooper
    • 2
  1. 1.EPSRC National Mass Spectrometry Service Centre (NMSSC), School of MedicineSwansea UniversitySwanseaUK
  2. 2.Department of ChemistryUniversity of LiverpoolLiverpoolUnited Kingdom

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