Semi-automated data processing of hydrogen exchange mass spectra using HX-Express

  • David D. Weis
  • John R. Engen
  • Ignatius J. Kass
Application Note


A Microsoft Excel utility, HX-Express, that significantly accelerates the analysis of hydrogen exchange mass spectrometry data is described. HX-Express generates deuterium uptake and peak width plots from peaks in mass spectral data. Data analysis is intentionally semi-automated, requiring that the user find the peaks to be analyzed. The peaks are entered in the form of x, y lists of m/z versus intensity or can be directly imported from Waters MassLynx software. Analysis of data with HX-Express provides the same results as manual data processing but is substantially faster. In addition to speed, HX-Express provides and preserves visual and numeric displays of the analysis process for quality control.


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Copyright information

© American Society for Mass Spectrometry 2006

Authors and Affiliations

  • David D. Weis
    • 1
  • John R. Engen
    • 1
  • Ignatius J. Kass
    • 2
  1. 1.Department of ChemistryUniversity of New MexicoAlbuquerqueUSA
  2. 2.Waters CorporationBeverlyUSA

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