Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry

  • Xu Zhang
  • Mark Scalf
  • Travis W. Berggren
  • Michael S. Westphall
  • Lloyd M. Smith
Articles

Abstract

Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein profiling is described in which mammalian cells are lysed directly in the MALDI matrix 2,5-dihydroxybenzoic acid (DHB) and mass analyzed using MALDI-time of flight (TOF). Using the unique MALDI mass spectral “fingerprint” generated in these analyses, it is possible to differentiate among several different mammalian cell lines. A number of techniques, including MALDI-post source decay (PSD), MALDI tandem time-of-flight (TOF-TOF), MALDI-Fourier transform ion cyclotron resonance (FTICR), and nanoflow liquid chromatography followed by electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) were employed to attempt to identify the proteins represented in the MALDI spectra. Performing a tryptic digestion of the supernatant of the cells lysed in DHB with subsequent LC-ESI-MS/MS analysis was by far the most successful method to identify proteins.

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Copyright information

© American Society for Mass Spectrometry 2006

Authors and Affiliations

  • Xu Zhang
    • 1
  • Mark Scalf
    • 1
  • Travis W. Berggren
    • 1
  • Michael S. Westphall
    • 1
  • Lloyd M. Smith
    • 1
  1. 1.Department of ChemistryUniversity of Wisconsin-MadisonMadisonUSA

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