Journal of the American Society for Mass Spectrometry

, Volume 16, Issue 9, pp 1481–1487 | Cite as

Clear evidence of fluorescence resonance energy transfer in gas-phase ions

  • Maxim Dashtiev
  • Vladimir Azov
  • Vladimir Frankevich
  • Ludwig Scharfenberg
  • Renato Zenobi


Fluorescence resonance energy transfer (FRET) is a distance-sensitive method that correlates changes in fluorescence intensity with conformational changes, for example, of biomolecules in the cellular environment. Applied to the gas phase in combination with Fourier transform ion cyclotron resonance mass spectrometry, it opens up possibilities to define structural/conformational properties of molecular ions, in the absence of solvent, and without the need for purification of the sample. For successfully observing FRET in the gas phase it is important to find suitable fluorophores. In this study several fluorescent dyes were examined, and the correlation between solution-phase and gas-phase fluorescence data were studied. For the first time, FRET in the gas phase is demonstrated unambiguously.


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Copyright information

© American Society for Mass Spectrometry 2005

Authors and Affiliations

  • Maxim Dashtiev
    • 1
  • Vladimir Azov
    • 1
  • Vladimir Frankevich
    • 1
  • Ludwig Scharfenberg
    • 1
  • Renato Zenobi
    • 1
  1. 1.Department of Chemistry, ETH HonggerbergSwiss Federal Institute of TechnologyZurichSwitzerland

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