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Pathways of Peptide Ion Fragmentation Induced by Vacuum Ultraviolet Light

  • Weidong Cui
  • Matthew S. Thompson
  • James P. Reilly
Articles

Abstract

One Hundred Fifty-Seven nm photodissociation of singly protonated peptides generates unusual distributions of fragment ions. When the charge is localized at the C-terminus of the peptide, spectra are dominated by x-, v-, and w-type fragments. When it is sequestered at the N-terminus, a- and d-type ions are overwhelmingly abundant. Evidence is presented suggesting that the fragmentation occurs via photolytic radical cleavage of the peptide backbone at the bond between the α- and carbonyl-carbons followed by radical elimination to form the observed daughter ions.

Keywords

Electron Capture Dissociation Post Source Decay Norrish Type Photodissociation Spectrum Post Source Decay Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Society for Mass Spectrometry 2005

Authors and Affiliations

  • Weidong Cui
    • 1
  • Matthew S. Thompson
    • 1
  • James P. Reilly
    • 1
  1. 1.Department of ChemistryIndiana UniversityBloomingtonUSA

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