Enhanced Osteogenesis of Nanosized Cobalt-substituted Hydroxyapatite
- 8 Downloads
Hydroxyapatite (HAp) is an extensively studied material with known biocompatible and osteoconductive properties in bone tissue reconstruction. The improvement of the osteogenetic potential of HAp has been tested through modification of its structure, by replacing Ca2+ ions with Co2+ ions. In our study, we comparatively analyze the osteogenetic potential of the synthesized HAp and Co2+-substituted HAp (HAp/Co) designed on the nano-scale with the aim of specifically stimulating osteogenesis in vivo. We present a quantitative study of the microscopic organization and structure of the newly formed tissue in a bone defect after 12 weeks and 24 weeks. A quantitative analysis of the calcium, magnesium and phosphorus content in the defect and its close environment was used to determine the deposition of minerals after bone reconstruction. The defect reconstructed with HAp/Co nanoparticles (Co2+ content 12 wt%) was filled with a new tissue matrix composed of dense collagen fibers containing centers of mineralization after 24 weeks. The mineral deposition rate was also higher when the defect was reconstructed with HAp/Co than when it was filled with pure HAp. A histological analysis confirmed that the alveolar bone, in which osteoporosis-induced defects were repaired using HAp/Co nanoparticles, was recuperated.
Keywordsadvanced osteogenesis cobalt-substituted hydroxyapatite nanoparticles histomorphometry
Unable to display preview. Download preview PDF.
- Thian E, Konishi T, Kawanobe Y, Lim P, Choong C, Ho B, Aizawa M. Zinc-substituted hydroxyapatite: A biomaterial with enhanced bioactivity and antibacterial properties. Journal of Materials Science: Materials in Medicine, 2013, 24, 437–445.Google Scholar
- Ignjatović N, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović Z, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. Journal of Materials Science: Materials in Medicine, 2013, 24, 343–354.Google Scholar
- Dempster D, Compston J, Drezner M, Glorieux F, Kanis J, Malluche H, Meunier P, Ott S, Recker R, Parfitt M. Standardized nomenclature, symbols, and units for bone histo-morphometry: A 2012 update of the report of the ASBMR histomorphometry nomenclature committee. Journal of Bone and Mineral Research, 2013, 28, 2–17.CrossRefGoogle Scholar
- Drašler B, Drobne D, Novak S, Valant J, Boljte S, Otrin L, Rappolt M, Sartori B, Iglič A, Kralj-Iglič V, Šuštar V. Makovec D, Gyergyek S, Hočevar M, Godec M, Zupanc J. Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes. International Journal of Nanomedicine, 2014, 9, 1559–1581.CrossRefGoogle Scholar