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Journal of Bionic Engineering

, Volume 5, Issue 4, pp 308–316 | Cite as

Microfabrication of Bubbular Cavities in PDMS for Cell Sorting and Microcell Culture Applications

  • Ut-Binh T. Giang
  • Michael R. King
  • Lisa A. DeLouiseEmail author
Article

Abstract

We describe a novel technique, low surface energy Gas Expansion Molding (GEM), to fabricate microbubble arrays in polydimethylsiloxane (PDMS) which are incorporated into parallel plate flow chambers and tested in cell sorting and microcell culture applications. This architecture confers several operational advantages that distinguish this technology approach from currently used methods. Herein we describe the GEM process and the parameters that are used to control microbubble formation and a Vacuum-Assisted Coating (VAC) process developed to selectively and spatially alter the PDMS surface chemistry in the wells and on the microchannel surface. We describe results from microflow image visualization studies conducted to investigate fluid streams above and within microbubble wells and conclude with a discussion of cell culture studies in PDMS.

Keywords

polydimethylsiloxane microfabrication cell culture cell sorting molding 

Nomenclature

Bubbular

the spheroidal geometry exhibited by the formation of microbubbles.

PDMS

polydimethylsiloxane polymer

Microbubbles

bubbular structures molded into PDMS with an opening(s) on the side planar to the PDMS surface.

GEM

gas expansion molding, a new technique developed to form microbubbles in PDMS.

VAC

vacuum-assisted coating, a new technique developed to coat the interior wall of microbubbles in PDMS.

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Copyright information

© Jilin University 2008

Authors and Affiliations

  • Ut-Binh T. Giang
    • 1
  • Michael R. King
    • 2
  • Lisa A. DeLouise
    • 1
    • 3
    Email author
  1. 1.Department of Biomedical EngineeringUniversity of RochesterRochesterUSA
  2. 2.Department of Biomedical EngineeringCornell UniversityIthacaUSA
  3. 3.Department of DermatologyUniversity of Rochester Medical CenterRochesterUSA

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