Separation and detection of the α- and β-chains of hemoglobin of a single intact red blood cell using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry

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Abstract

A single intact red blood cell (erythrocyte) was injected into a capillary electrophoresis column, and following in-capillary lysing the α- and β-chains of the hemoglobin (∼450 amol) were separated and detected using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry. The mass spectra of the electropherogram peaks of the α and β chains showed identifiable peaks corresponding to multiply protonated and sodiated α- and β-chains of hemoglobin.

Keywords

Capillary Electrophoresis Carbonic Anhydrase Capillary Inlet Intact Erythrocyte Capillary Electrophoresis Instrument 
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References

  1. 1.
    Swanek, F. D.; Ferris, S. S.; Ewing, A. G. Capillary electrophoresis for the analysis of single cells: electrochemical, mass spectrometric, and radiochemical detection. In Handbook of Capillary Electrophoresis; Landers, J. P., Ed.; CRC: Boca Raton, FL; 1997; Chap 17.Google Scholar
  2. 2.
    Hofstadler, S. A.; Severs, J. C.; Smith, R. D.; Swanek, F. D.; Ewing, A. G. Rapid Commun. Mass Spectrom. 1996, 10, 919.CrossRefGoogle Scholar
  3. 3.
    Smith, R. D.; Barinaga, C. J.; Udseth, H. R. Anal. Chem. 1988, 60, 1948.CrossRefGoogle Scholar
  4. 4.
    Lee, E. D.; Muck, W.; Henion, J. D.; Covey, T. R. Biomed. Env. Mass Spectrom. 1989, 18, 844.CrossRefGoogle Scholar
  5. 5.
    Foret, F.; Thompson, T. J.; Vouros, P.; Karger, B. L. Anal. Chem. 1994, 66, 4450.CrossRefGoogle Scholar
  6. 6.
    Cai, J.; Henion, J. J. Chromatogr. A 1995, 703, 667.CrossRefGoogle Scholar
  7. 7.
    Severs, J. C.; Harms, A. C.; Smith, R. D. Rapid Commun. Mass Spectrom. 1996, 10, 1175.CrossRefGoogle Scholar
  8. 8.
    Figeys, D.; Oostveen, I. V.; Ducret, A.; Aebersold, R. Anal. Chem. 1996, 68, 1822.CrossRefGoogle Scholar
  9. 9.
    Cao, P.; Moini, M. J. Am. Soc. Mass Spectrom. 1997, 8, 561.CrossRefGoogle Scholar
  10. 10.
    Yamashita, M.; Fenn, J. B. J. Phys. Chem. 1984, 88, 4451.CrossRefGoogle Scholar
  11. 11.
    Whitehouse, C. M.; Dreyer, R. N.; Yamashita, M.; Fenn, J. B. Anal. Chem. 1985, 57, 675.CrossRefGoogle Scholar
  12. 12.
    Siethoff, C.; Nigge, W.; Linscheid, M. Anal. Chem. 1998, 70, 1357.CrossRefGoogle Scholar
  13. 13.
    Cao, P.; Moini, M. Rapid Commun. Mass Spectrom. 1998, 12, 864.CrossRefGoogle Scholar
  14. 14.
    Cao, P.; Moini, M. Electrophoresis 1998, 19, 2200.CrossRefGoogle Scholar
  15. 15.
    Cao, P.; Moini, M. J. Am. Soc. Mass Spectrom. 1998, 9, 1081.CrossRefGoogle Scholar
  16. 16.
    Fang, L.; Zhang, R.; Williams, E. R.; Zare, R. N. Anal. Chem. 1994, 66, 3696.CrossRefGoogle Scholar
  17. 17.
    Wu, J. T.; Qian, M. M. G.; Liu, L.; Lubman, D. M. Anal. Chem. 1996, 68, 3388.CrossRefGoogle Scholar
  18. 18.
    Moseley, M. A.; Jorgenson, W. J.; Shabanowitz, J.; Hunt, D. F.; Tomer, K. B. J. Am. Soc. Mass Spectrom. 1992, 3, 289.CrossRefGoogle Scholar
  19. 19.
    Hogan, B. L.; Yeung, E. S. Anal. Chem. 1992, 64, 2841.CrossRefGoogle Scholar
  20. 20.
    Li, L.; Golding, R. E.; Whittal, R. M. J. Am. Chem. Soc. 1996, 118, 11662.CrossRefGoogle Scholar
  21. 21.
    Pennell, R. B. Composition of normal human red cells. In The Red Blood Cell, Vol. I; Surgenor, D. M., Ed.; Academic, New York, 1974, p 93.Google Scholar

Copyright information

© American Society for Mass Spectrometry 1999

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of Texas at AustinAustin

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