Origin of product ions in the MS/MS spectra of peptides in a quadrupole ion trap

  • Richard W. Vachet
  • Kenneth L. Ray
  • Gary L. Glish
Short Communication


Stored waveform inverse Fourier transform and double resonance techniques have been used in conjunction with a quadrupole ion trap to study the dissociation patterns of peptide ions. These experiments provide insight into the origin of individual product ions in an MS/MS spectrum. Results show for a series of leucine enkephalin analogues with five amino acid residues that the b 4 ion is the main product ion through which many other product ions arise. It was also observed that the percentage of the a 4 product ions that are formed directly from the protonated molecule (M+H)+ depends on the nature of the fourth amino acid residue. In addition, it was determined that in the peptides studied here lower series b ions (e.g., b 3 arise from direct dissociation of higher series b ions (e.g., b 4 only about 50% of the time.


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Copyright information

© American Society for Mass Spectrometry 1998

Authors and Affiliations

  • Richard W. Vachet
    • 1
  • Kenneth L. Ray
    • 1
  • Gary L. Glish
    • 1
  1. 1.Department of ChemistryUniversity of North CarolinaChapel HillUSA

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