Method for screening peptide fragment ion mass spectra prior to database searching

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Abstract

A methodology is described for screening fragment ion spectra of peptides prior to database searching for protein identification. A software routine written in the Perl programming language was used to analyze data from previous Sequest database searches and develop a set of statistical descriptors that could be used to identify spectra not likely to yield useful results in a database search. A second Perl program used an evolutionary algorithm to optimize the criteria for each statistical descriptor and generate a formula for determining spectral quality. This formula was used by a third Perl program to screen data sets from four independent liquid chromatography tandem mass spectrometry runs. On the average, use of the screening program reduced the time required for a database search by 1/2 with little loss of useful information from the database search results.

Keywords

Perl Program Good Spectrum Sequest Search Database Search Result Base Peak Intensity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© American Society for Mass Spectrometry 2000

Authors and Affiliations

  • Roger E. Moore
    • 1
  • Mary K. Young
    • 1
  • Terry D. Lee
    • 1
  1. 1.Division of ImmunologyBeckman Research Institute of the City of HopeDuarte

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