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Structural determination of picomole amounts of phospholipids via electrospray ionization tandem mass spectrometry

  • Xianlin Han
  • Richard W. Gross
Articles

Abstract

The remarkable sensitivity of electrospray ionization was exploited to achieve great increases in the sensitivity of tandem mass spectrometric analyses of phospholipids derived from both synthetic and biologic sources. Herein, we demonstrate that (1) product-ion spectra after electrospray ionization can be obtained easily by utilizing ≤ 5 pmol of phospholipid with a mass-selected window of less than 2 mass units, (2) the low energy inherent in the electrospray ionization method facilitates analysis of labile molecular ions that are not easily detected with the relatively high energy employed during fast-atom bombardment desorption, and (3) collision-induced dissociation of precursor ions generated from electrospray ionization often resulted in novel product-ion patterns. Collectively, these results underscore the utility of electrospray ionization tandem mass spectroscopy for the structural determination of diminutive amounts of phospholipids.

Keywords

Neutral Loss Collisional Activation Carboxylate Anion Ethanolamine Glycerophospholipids Choline Glycerophospholipids 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Han, X.; Gross, R. W. Proc. Natl. Acad. Sci. USA 1994, 91, 10635–10639.CrossRefGoogle Scholar
  2. 2.
    Weintraub, S. T.; Pinckard, R. N.; Hail, M. Rapid Commun. Mass Spectrom. 1991, 5, 309–311.CrossRefGoogle Scholar
  3. 3.
    Kerwin, J. L.; Tuininga, A.; Ericsson, L. H. J. Lipid Res. 1994, 35, 1102–1114.Google Scholar
  4. 4.
    Kim, H.-Y.; Wang, T.-C. L.; Ma, Y.-C. Anal. Chem. 1994, 66, 3977–3982.CrossRefGoogle Scholar
  5. 5.
    Cole, M. J.; Enke, C. G. Anal. Chem. 1991, 63, 1032–1038.CrossRefGoogle Scholar
  6. 6.
    Kayganich, K. A.; Murphy, R. C. Anal. Chem. 1992, 64, 2965–2971.CrossRefGoogle Scholar
  7. 7.
    Bryant, D. K.; Orlando, R. C.; Fenselau, C. Anal. Chem. 1991, 63, 1110–1114.CrossRefGoogle Scholar
  8. 8.
    Jensen, N. J.; Gross, M. L. Mass Spectrom. Rev. 1988, 7, 41–69.CrossRefGoogle Scholar
  9. 9.
    Benfenati, E.; Reginato, R. Biomed. Mass Spectrom. 1985, 12, 643–651.CrossRefGoogle Scholar
  10. 10.
    Ayanoglu, E.; Wegmann, A.; Pilet, O.; Marbury, G. D.; Hass, J. R.; Djerassi, C. J. Am. Chem. Soc. 1984, 106, 5246–5251.CrossRefGoogle Scholar
  11. 11.
    Matsubara, T.; Hayashi, A. Prog. Lipid Res. 1991, 30, 301–322.CrossRefGoogle Scholar
  12. 12.
    Patton, G. M.; Fasulo, J. M.; Robins, S. J. J. Lipid Res. 1982, 23, 190–196.Google Scholar
  13. 13.
    Gross, R. W. Biochemistry 1984, 23, 158–165.CrossRefGoogle Scholar
  14. 14.
    Han, X.; Zupan, L. A.; Hazen, S. L.; Gross, R. W. Anal. Biochem. 1992, 200, 119–124.CrossRefGoogle Scholar
  15. 15.
    Fink, K. L.; Gross, R. W. Circ. Res. 1984, 55, 585–594.Google Scholar
  16. 16.
    Jensen, N. J.; Tomer, K. B.; Gross, M. L. Lipids 1986, 21, 580–588.CrossRefGoogle Scholar
  17. 17.
    Münster, H.; Budzikiewicz, H. Biol. Chem. Hoppe-Seyler 1988, 369, 303–308.Google Scholar
  18. 18.
    Kayganich, K.; Murphy, R. C. J. Am. Soc. Mass Spectrom. 1991, 2, 45–54.CrossRefGoogle Scholar
  19. 19.
    Zirrolli, J. A.; Clay, K. L.; Murphy, R. C. Lipids 1991, 26, 1112–1116.CrossRefGoogle Scholar
  20. 20.
    Huang, Z.-H.; Gage, D. A.; Sweeley, C. C. J. Am. Soc. Mass Spectrom. 1992, 3, 71–78.CrossRefGoogle Scholar
  21. 21.
    Murphy, R. C. Mass Spectrometry of Lipid Plenum Press, San Diego, 1993; pp 290.Google Scholar
  22. 22.
    Kim, H.-Y.; Salem, N. Jr. Anal. Chem. 1986, 58, 9–14.CrossRefGoogle Scholar
  23. 23.
    Haroldsen, P. E.; Gaskell, S. J. Biomed. Environ. Mass Spectrom. 1989, 18, 439–444.CrossRefGoogle Scholar
  24. 24.
    Jensen, N. J.; Tomer, K. B.; Gross, M. L. Lipids 1987, 22, 480–489.CrossRefGoogle Scholar
  25. 25.
    Heller, D. N.; Murphy, C. M.; Cotter, R. J.; Fenselau, C.; Uy, O. M. Anal. Chem. 1988, 60, 2787–2791.CrossRefGoogle Scholar

Copyright information

© American Society for Mass Spectrometry 1995

Authors and Affiliations

  • Xianlin Han
    • 1
  • Richard W. Gross
    • 1
  1. 1.Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Chemistry, and Molecular Biology & PharmacologyWashington University School of MedicineSt. LouisUSA

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