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Combined capillary gas chromatography/ion trap mass spectrometry quantitative methods using labeled or unlabeled internal standards

  • R. J. Strife
  • J. R. Simms
  • M. P. Lacey
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Abstract

Three methods of operating an ion trap mass spectrometer (ITMS) were investigated for the determination of quantitative information by combined capillary GC/MS and GC/MS/MS. Separate regression Lines for a hexynone drug were examined by using bath unlabeled and stable-isotope-labeled internal standards. The ITMS modes of operation examined were (1) the full-scan, rf-voltage-only mode, useful on ion trap detectors as well as on the ITMS, (2) a scan using combined rf and dc voltages (rf/dc) for mass-selective storage analyses, and (3) rf/dc followed by collision-induced dissociation, Results of over 200 analyses in the 0.1–10 ng on-column range, with 5 ng of internal standard, showed that the unlabeled internal standard, separated by retention time on the capillary GC column, gave the best relative standard deviatiod (less than 5% over the range) and linear correlations (r 2 typically > 0.9992).

Keywords

Automatic Gain Control Ionization Time Miami Valley Laboratory High Split Ratio Standard Quadrupole 
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References

  1. 1.
    Dawson, P. H., Ed. Quadrupole Mass Spectrometry and Its Applications; Elsevier: New York, 1976.Google Scholar
  2. 2.
    March, R. E.; Hughes, R. J.; Todd, J. F. J. Quadrupole Storage Mass Spectrometry; Wiley: New York, 1989.Google Scholar
  3. 3.
    Louris, J. N.; Cooks, R. G.; Syka, J. E. P.; Kelly, P. E.; Stafford, G. C. Anal. Chem. 1987, 59, 1677–1685.CrossRefGoogle Scholar
  4. 4.
    Stafford, G. C.; Taylor, D. M.; Bradshaw, S. C.; Syka, J. E. P.; Ulrich, M. Presented at the 35th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, Col., May 24–29, 1987.Google Scholar
  5. 5.
    Yost, R. A.; McClennen, W.; Snyder, A. P. Presented at the 35th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, Col., May 24–29,1987.Google Scholar
  6. 6.
    Strife, R. J.; Simms, J. R. Anal. Chem. 1989, 61, 2316–2319.CrossRefGoogle Scholar
  7. 7.
    Tucker, D. B.; Hameister, C. H.; Bradshaw, S. C.; Hoekaman, D. J.; Weber-Grabau, M. Presented at the 36th ASME Conference on Mass Spectrometry and Allied Topics, San Francisco, Cal., June 5–10, 1988.Google Scholar
  8. 8.
    Strife, R. J.; Kelley, P. E.; Weber-Grabau, M. Rapid Commun. Mass Spectrom. 1988, 2, 105–109.CrossRefGoogle Scholar
  9. 9.
    Technical Report 908, Finnigan-MAT, San Jose, Cal., 1988.Google Scholar
  10. 10.
    Dobson, R. L.; Neal, D. M.; DeMark, B. R.; Ward, S. Presented at the 37th ASMS Conference on Mass Spectrometry and Allied Topics, Miami Beach, Fla., May 22–26, 1989.Google Scholar
  11. 11.
    Doyle, M. J.; Eichold, T. Biomed. Environ. Mass Spectrom. 1990. in press.Google Scholar
  12. 12.
    Strife, R. J.; Keller, P. R. Org. Mass Spectrom. 1989, 24, 201–204.CrossRefGoogle Scholar
  13. 13.
    Brodbelt, J. S.; Louris, J. N.; Cooks, R. G. Anal. Chem. 1984, 59, 1278–1285.CrossRefGoogle Scholar
  14. 14.
    Keough, T.; Strife, R. J.; Rodriguez, P. A.; Sanders, R. A. J. Chromatogr. 1984, 312, 450–455.CrossRefGoogle Scholar

Copyright information

© American Society for Mass Spectrometry 1990

Authors and Affiliations

  • R. J. Strife
    • 1
  • J. R. Simms
    • 1
  • M. P. Lacey
    • 1
  1. 1.Corporate Research Division, Miami Valley LaboratoriesThe Procter & Gamble CompanyCincinnatiUSA

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