Investigations of gas-phase lithium-peptide adducts: Tandem. mass spectrometry and semiempirical studies

  • Julie A. Leary
  • Zhongrui Zhou
  • Sherri A. Ogden
  • Todd D. Williams
Article

Abstract

Tandem mass spectrometry using a hybrid mass spectrometer of BEqQ geometry was used to investigate the gas-phase formation of the [An+ Li − H)+ ion from lithium-peptide adducts. High resolution mass measurements as well as precursor and product ion scans of five peptides indicate that one source of [An+ Li − H) arises from [An+ Li]+. Semiempirical calculations (MNOO) and metastable ion decomposition studies of the peptide Gly-Gly-Gly show that the lithium ion prefers to coordinate to the three internal carbonyls of the neutral molecule to give a species that is energetically more stable than the lithiated zwitterion by 305 kJ/mol. Theoretical and experimental evidence suggest that the monolithiated precursor ion population may be a distribution of structural isomers. (J Am Soc Mass Spectrom 1990, 1, 473-480)

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Copyright information

© American Society for Mass Spectrometry 1990

Authors and Affiliations

  • Julie A. Leary
    • 1
  • Zhongrui Zhou
    • 1
  • Sherri A. Ogden
    • 1
  • Todd D. Williams
    • 1
  1. 1.College of ChemistryUniversity of CaliforniaBerkeleyUSA

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