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A high-throughput sequence determination for one cyclic peptide immobilized on a single bead by the one-pot cyanidation followed by MALDI-TOF–MS/MS for discovery of interacting peptides

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Abstract

One cyclic peptide immobilized on one gel-type bead has been employed for the discovery of both interacting peptides and/or medicinal medium-sized molecules. Although high-throughput characterization of recognized peptides has been a bottleneck, here, we describe direct liberation from beads by a one-pot reaction using 2-nitro-5-thiocyanatobenzoic acid followed by mass spectrometry to realize faster and routine sequencing of the peptide on the beads. This is useful for the investigation of protein–protein interactions as well as discovery of drug candidates.

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Fig. 1
Scheme 1

Modified from Jacobson9

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Authors and Affiliations

  1. HiPep Laboratories, Nakatsukasa-Cho 486-46, Kamigyo-Ku, Kyoto, 602-8158, Japan

    Takeshi Kasama & Kiyoshi Nokihara

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  1. Takeshi Kasama
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  2. Kiyoshi Nokihara
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Correspondence to Kiyoshi Nokihara.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Kasama, T., Nokihara, K. A high-throughput sequence determination for one cyclic peptide immobilized on a single bead by the one-pot cyanidation followed by MALDI-TOF–MS/MS for discovery of interacting peptides. ANAL. SCI. (2024). https://doi.org/10.1007/s44211-024-00594-8

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  • DOI: https://doi.org/10.1007/s44211-024-00594-8

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