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Fluorescent immunoassay for chloramphenicol based on the label-free polyadenine-mediated spherical nucleic acids triggered signal amplification

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Abstract

A fluorescent immunosorbent assay incorporating signal amplification away from the surface of spherical nucleic acid (SNA) was developed for the detection of chloramphenicol (CAP). Through the conjugation of antibodies and poly-adenine (polyA) DNA onto the surface of gold nanoparticles (AuNPs), the fabrication of the nano-immunoprobe was achieved in a more straightforward and cost-effective manner. Moreover, a strategy utilizing the hybridization chain reaction (HCR) in the amplification step was devised, with particular attention given to the enzyme inhibition associated with SNA. The results demonstrated good performance on CAP detection with a linear range of 0.01–5 ng/L with a detection limit of 0.005 ng/L. The significance of this work mainly lies in the polyA-SNA-based immunoprobe and the thoughtful design to prevent enzyme inhibition.

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Acknowledgements

This work was supported by the Jiangsu Provincial Health Commission Research Programme (No. M2021078), Jiangsu Provincial Health Commission Scientific Research Fund Schistosomiasis-Endemic-Parasitic Disease Prevention Project (x202314), Jiangsu Province Key Research and Development Program (BE2022837).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Weiwei Shen & Yahui Guo

  2. State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co., Ltd., Shanghai, People’s Republic of China

    Qing Hong & Zhenmin Liu

  3. Department of Food Safety and Assessment, Jiangsu Provincial Center for Disease Control and Prevention; Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Province Engineering Research Center of Health Emergency, Nanjing, China

    Xiang Huo & Yijing Zhou

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  1. Weiwei Shen
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  2. Qing Hong
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  3. Xiang Huo
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  4. Yijing Zhou
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  5. Yahui Guo
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Contributions

Weiwei Shen: Investigation, experiment, writing—original draft. Yahui Guo: Writing—review and editing, supervision.

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Correspondence to Yahui Guo.

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Shen, W., Hong, Q., Huo, X. et al. Fluorescent immunoassay for chloramphenicol based on the label-free polyadenine-mediated spherical nucleic acids triggered signal amplification. ANAL. SCI. (2024). https://doi.org/10.1007/s44211-024-00565-z

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