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Sandwich-type electrochemical immunosensing of CA125 by using nanoribbon-like Ti3C2Tx MXenes and toluidine blue/UIO-66-NH2

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Abstract

CA125 (carbohydrate antigen 125) is an important biomarker of ovarian cancer, so developing effective method for its detection is of great significance. In the present work, a novel sandwich-like electrochemical immunosensor (STEM) of CA125 was constructed by preparing nanoribbon-like Ti3C2Tx MXenes (Ti3C2TxNR) to immobilize primary antibody (PAb) of CA125 and UIO-66-NH2 MOFs structure to immobilize second antibody (SAb) and electroactive toluidine blue (Tb) probe. In this designed STEM assay, the as-prepared Ti3C2TxNR nanohybrid offers the advantages in large surface area and conductivity as carrier, and UIO-66-NH2 provided an ideal platform to accommodate SAb and a large number of Tb molecules as signal amplifier. In the presence of CA125, the peak currents of Tb from the formed STEM structure increase with the increase of CA125 level. After optimizing the related control conditions, a wide linear range (0.2–150.0 U mL−1) and a very low detection limit (0.05 U mL−1) of CA125 were achieved. It’s thus expected the developed STEM strategy has important applications for the detection of CA125.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This work was supported by the Natural Science Foundation of Fujian Province (Grant#: 2020J011302, 2020J011299 and 2022J011481), and Zhangzhou Natural Science Foundation Project (Grant#: Z2020J05).

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Author notes

  1. Xuqiao Mei, Zhenhua Zeng and Wenxin Xu have contributed equally to this work.

Authors and Affiliations

  1. Department of Clinical Laboratory, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian, China

    Xuqiao Mei, Zhenhua Zeng, Huicong Yang, Yuanhai Zheng, Haimin Gao, Chuncai Wu, Yanping Zheng, Qiaoli Xu, Guowei Wang, Yuhuang Xu & Ayang Wu

  2. Collaborative Innovation Center for Translation Medical Testing and Application Technology, Zhangzhou Health Vocational College, Zhangzhou, Fujian, China

    Wenxin Xu

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  1. Xuqiao Mei
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Correspondence to Ayang Wu.

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Mei, X., Zeng, Z., Xu, W. et al. Sandwich-type electrochemical immunosensing of CA125 by using nanoribbon-like Ti3C2Tx MXenes and toluidine blue/UIO-66-NH2. ANAL. SCI. 40, 1081–1087 (2024). https://doi.org/10.1007/s44211-024-00528-4

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