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A pH-mediated field amplification sample stacking technique based on portable microchip electrophoresis heavy metal ion detection system

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Abstract

We built a portable microchip electrophoresis heavy metal ion detection system and proposed a pH-mediated field amplified sample stacking (pH-mediated FASS) online preconcentration method. The pH-mediated FASS focuses and stacks heavy metal cations by controlling electrophoretic mobilities with a pH change between the analyte and the background electrolyte (BGE) in solution to improve the detection sensitivity of the system. We optimized and adjusted sample matrix solution (SMS) ratios and pH to create concentration and pH gradients for SMS and BGE. Furthermore, we optimize the microchannel width to improve the preconcentration effect further. The system and method analyzed soil leachates polluted with heavy metals and separated Pb2+ and Cd2+ within 90 s, obtaining their levels at 58.01 mg/L and 4.91 mg/L with sensitivity enhancement factors (SEF) of 26.40 and 43.73. Compared with inductively coupled plasma atomic emission spectrometry (ICP-AES), the detection error of the system was less than 8.80%.

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Acknowledgements

This research was supported by Guangxi Bagui Scholars Project (No. C3010099204) and the Opening Project of the National Enterprise Technology Center of Guangxi Bossco Environmental Protection Technology Co (Item No. GXU-BFY-2020-012).

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Authors and Affiliations

  1. School of Mechanical Engineering, Guangxi University, Nanning, 530004, China

    Yaping Liu, Zihao Lu, Jianjiao Wang & Hui You

  2. Guangxi Bossco Environmental Protection Technology Co., Ltd., Nanning, 530007, China

    Jian Su & Chengwu Mo

  3. School of Electrical Engineering, Guangxi University, Nanning, 530004, China

    Wenhe He

  4. School of Computer, Electronics and Information, Guangxi University, Nanning, 530004, China

    Cuimin Sun

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  1. Yaping Liu
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  2. Wenhe He
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Correspondence to Hui You.

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Liu, Y., He, W., Lu, Z. et al. A pH-mediated field amplification sample stacking technique based on portable microchip electrophoresis heavy metal ion detection system. ANAL. SCI. 39, 1475–1482 (2023). https://doi.org/10.1007/s44211-023-00364-y

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  • DOI: https://doi.org/10.1007/s44211-023-00364-y

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