Skip to main content

Advertisement

SpringerLink
Log in

Label-free photoelectrochemical immunosensing of α-fetoprotein based on Eu-TiO2 nanocomposites sensitized with dye-encapsulated HMA

  • Original Paper
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

In this study, a sensitive photoelectrochemical immunosensor with dye-enhanced anodic photocurrent response was proposed for sensitive detection of α-fetoprotein (AFP). Specifically, europium-doped TiO2 (Eu-TiO2) was used as the photoelectrochemical functional material and coated onto indium tin oxide (ITO) electrode. Doxorubicin (DOX) as an excellent fluorescent dye was encapsulated in the hydrophobically modified alginate (HMA). Then the dye-loaded HMA was modified onto the surface of Eu-TiO2 to further sensitize the photocurrent response. The results showed that the photoelectrical signal was enhanced and stabilized due to the effect of sensitization of DOX on Eu-TiO2 material. The constructed PEC sensor revealed a good linear response to AFP antigen ranging from 0.5 to 100 ng/mL with a detection limit of 0.41 pg/mL. The clinical patient’s serum test results obtained from the proposed PEC immunosensor were consistent with those obtained from the commercial electrochemilunescence assay. The proposed PEC sensing method could be a promising analytical tool for the detection of AFP in clinical analysis.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Data availability

The authors declare that the datas are availability in this paper.

References

  1. W.W. Zhao, J.J. Xu, H.Y. Chen, Anal. Chem. 90(1), 615–627 (2018)

    Article  CAS  PubMed  Google Scholar 

  2. W.W. Zhao, J.J. Xu, H.Y. Chen, Chem. Rev. 114(15), 7421–7441 (2014)

    Article  CAS  PubMed  Google Scholar 

  3. W.W. Zhao, R. Chen, P.P. Dai, X.L. Li, J.J. Xu, H.Y. Chen, Anal. Chem. 86(23), 11513–11516 (2014)

    Article  CAS  PubMed  Google Scholar 

  4. G.C. Fan, H. Zhu, D. Du, J.R. Zhang, J.J. Zhu, Y. Lin, Anal. Chem. 88(6), 3392–3399 (2016)

    Article  CAS  PubMed  Google Scholar 

  5. Y.C. Zhu, N. Zhang, Y.F. Ruan, W.W. Zhao, J.J. Xu, H.Y. Chen, Anal. Chem. 88(11), 5626–5630 (2016)

    Article  CAS  PubMed  Google Scholar 

  6. X.P. Liu, J.S. Chen, C.J. Mao, H.L. Niu, J.M. Song, B.K. Jin, Biosens. Bioelectron. 116, 23–29 (2018)

    Article  CAS  PubMed  Google Scholar 

  7. X. Qin, Q. Wang, L. Geng, X. Shu, Y. Wang, Talanta 197, 28–35 (2019)

    Article  CAS  PubMed  Google Scholar 

  8. Y. Liu, R. Li, P. Gao, Y. Zhang, H. Ma, J. Yang, B. Du, Q. Wei, Biosens. Bioelectron. 65, 97–102 (2015)

    Article  CAS  PubMed  Google Scholar 

  9. D.S. Zhen, M. Guo, X.F. Li, H.L. Mao, C.A. Li, J.L. Li, Y. Liu, J. Nanosci, Nanotechnol. 19(12), 7758–7763 (2019)

    CAS  Google Scholar 

  10. S. Wu, H. Song, J. Song, C. He, J. Ni, Y. Zhao, X. Wang, Anal. Chem. 86(12), 5922–5928 (2014)

    Article  CAS  PubMed  Google Scholar 

  11. W. Tu, Y. Dong, J. Lei, H. Ju, Anal. Chem. 82(20), 8711–8716 (2010)

    Article  CAS  PubMed  Google Scholar 

  12. S. Suarasan, A.M. Craciun, E. Licarete, M. Focsan, K. Magyari, S. Astilean, A.C.S. Appl, Mater. Interfaces 11(8), 7812–7822 (2019)

    Article  CAS  Google Scholar 

  13. X. Gao, Z. Yu, B. Liu, J. Yang, X. Yang, Y. Yu, Eur. Poly. J 133, 109779 (2020)

    Article  CAS  Google Scholar 

  14. P. Chen, Z. Wang, S. Wang, M. Lyu, M. Hao, M. Ghasemi, M. Xiao, J.H. Yun, Y. Bai, L. Wang, Nano Energy 69, 104392 (2020)

    Article  CAS  Google Scholar 

  15. C.Y. Huang, R.T. Guo, W.G. Pan, J.Y. Tang, W.G. Zhou, H. Qin, X.Y. Liu, P.Y. Jia, J. CO2 Util. 26, 487–495 (2018)

    Article  CAS  Google Scholar 

  16. Z. Guo, T. Hao, J. Duan, S. Wang, D. Wei, Talanta 89, 27–32 (2012)

    Article  CAS  PubMed  Google Scholar 

  17. M. Miyake, K. Sugano, H. Sugino, K. Imai, E. Matsumoto, K. Maeda, S. Fukuzono, H. Ichikawa, K. Kawashima, K. Hirabayashi, T. Kodama, H. Fujimoto, T. Kakizoe, Y. Kanai, K. Fujimoto, Y. Hirao, Cancer Sci. 101(1), 250–258 (2010)

    Article  CAS  PubMed  Google Scholar 

  18. C.J.P. Smith, P.C. Kelleher, BBA-Rev. Cancer 605(1), 1–32 (1980)

    CAS  Google Scholar 

  19. H. Yokoo, T. Kondo, K. Fujii, T. Yamada, S. Todo, S. Hirohashi, Hepatology 40(3), 609–617 (2004)

    Article  CAS  PubMed  Google Scholar 

  20. S. Sandoval, J. Yang, J.G. Alfaro, A. Liberman, M. Makale, C.E. Chiang, I.K. Schuller, A.C. Kummel, W.C. Trogler, Chem. Mater. 24(21), 4222–4230 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. J.L. Xochihua Juan, C. Solis Maldonado, R.A. Luna Sánchez, O.J. Enciso Díaz, M.R. Rojas Ronquillo, L. Sandoval-Rangel, N. Pineda Aguilar, N.A. Ramos Delgado, D.X. Martínez-Vargas, Catal. Today 394–396, 304–313 (2022)

    Article  Google Scholar 

  22. Y. Zhou, Y. Shi, F.-B. Wang, X.-H. Xia, Anal. Chem. 91(4), 2759–2767 (2019)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We appreciated the finance supports from the Natural Science Foundation of Shandong Province of China (ZR2019MB051), Shandong Provincial Key Research and Development Program (2018GGX102001).

Author information

Authors and Affiliations

  1. Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, People’s Republic of China

    Kun Wang, Xue Gao & Xiaoyan Yang

  2. Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, People’s Republic of China

    Jianfeng Chen

Authors
  1. Kun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xue Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianfeng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaoyan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiaoyan Yang.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 111 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, K., Gao, X., Chen, J. et al. Label-free photoelectrochemical immunosensing of α-fetoprotein based on Eu-TiO2 nanocomposites sensitized with dye-encapsulated HMA. ANAL. SCI. 39, 1171–1178 (2023). https://doi.org/10.1007/s44211-023-00326-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s44211-023-00326-4

Keywords

Search

Navigation