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Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model

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Abstract

Bioassays using three-dimensional (3D) tissue models offer several advantages over 2D culture assays because they can reproduce the structure and function of native tissues. In this study, we used our newly designed gelatin device to generate a miniature 3D model of human oral squamous cell carcinoma with stroma and blood vessels. To enable air–liquid interface culture, we conceived a new device structure in which three wells were lined up and separated by a dividing thread; the wells could be connected by removing the dividing thread. Cells were seeded in the center well with the dividing thread to form a multilayer, followed by the supply of media from the side wells after thread removal. Human oral squamous cell carcinoma (HSC-4) cells, human umbilical vein endothelial cells (HUVECs), and normal human dermal fibroblasts (NHDFs) were successfully cocultured, resulting in structures that mimicked 3D-cancer tissues. This 3D-cancer model was subjected to an X-ray sensitivity assay, followed by the evaluation of DNA damage using confocal microscopy and section-scanning electron microscopy.

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All data generated or analysed during this study are included in this article.

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Acknowledgements

This work was partially supported by Grant-in-Aid for Scientific Research (JSPS, KAKENHI; Grant No. 21H01970, 21K18988). This work was conducted at Central Research Laboratory, Okayama University Medical School, Japan.

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Authors and Affiliations

  1. Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo, Tokyo, 112-8681, Japan

    Tomoka Bessho, Tomoko Takagi & Kae Sato

  2. Neutron Therapy Research Center, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan

    Kazuyo Igawa

Authors
  1. Tomoka Bessho
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  2. Tomoko Takagi
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  3. Kazuyo Igawa
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  4. Kae Sato
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Contributions

Conceived and designed the experiments: KS. Performed the experiments: TB, TT, KI. Wrote the paper: TB, TT, KS.

Corresponding author

Correspondence to Kae Sato.

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The authors declare no competing of interest. The authors have no relevant financial or non-financial interests to disclose.

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Bessho, T., Takagi, T., Igawa, K. et al. Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model. ANAL. SCI. 39, 771–778 (2023). https://doi.org/10.1007/s44211-023-00308-6

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  • DOI: https://doi.org/10.1007/s44211-023-00308-6

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