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Construction of MOF@COF composite-based electrochemical aptasensor for detection of Staphylococcus aureus

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Abstract

In this work, a biological metal–organic framework@conductive covalent organic framework composite (bio-MOF@con-COF, denoted as Zn-Glu@PTBD-COF, here, Glu indicates L-glutamic acid, PT indicates 1,10-phenanthroline-2,9-dicarbaldehyde, and BD indicates benzene-1,4-diamine) was prepared and used as sensing material to fabricate aptasensor for trace detection of Staphylococcus aureus (SA). The Zn-Glu@PTBD-COF integrates the mesoporous structure and abundant defects of the MOF framework, the excellent conductivity of the COF framework, and high stability of the composite, providing abundant active sites to effectively anchor aptamers. As a result, the Zn-Glu@PTBD-COF-based aptasensor shows high sensitivity to detect SA via specific recognition between aptamer and SA, as well as the formation of aptamer–SA complex. Low detection limits of 2.0 and 1.0 CFU·mL−1 are deduced from the electrochemical impedance spectroscopy and differential pulse voltammetry within a wide linear range of 10–108 CFU·mL−1 for SA, respectively. The Zn-Glu@PTBD-COF-based aptasensor also shows good selectivity, reproducibility, stability, regenerability, and applicability for real milk and honey samples. Therefore, the Zn-Glu@PTBD-COF-based aptasensor will be promising for fast screening of foodborne bacteria in food service industry.

Graphical abstract

Zn-Glu@PTBD-COF composite was prepared and used as sensing material to fabricate aptasensor for trace detection of Staphylococcus aureus (SA). Low detection limits of 2.0 and 1.0 CFU·mL−1 are deduced from the electrochemical impedance spectroscopy and differential pulse voltammetry within a wide linear range of 10–108 CFU·mL−1 for SA, respectively. The Zn-Glu@PTBD-COF-based aptasensor also shows good selectivity, reproducibility, stability, regenerability, and applicability for real milk and honey samples.

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Data Availability

The authors declare that all the experimental data are available.

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Acknowledgements

This work was supported by the Henan Provincial Science and Technology Research Project (222102310493).

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Authors and Affiliations

  1. School of Mechanical Engineering, Henan University of Engineering, Zhengzhou, 451191, People’s Republic of China

    Yubo Meng, Zongzheng Ma & Yuchun Huang

  2. College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, People’s Republic of China

    Yingpan Song

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Meng, Y., Ma, Z., Huang, Y. et al. Construction of MOF@COF composite-based electrochemical aptasensor for detection of Staphylococcus aureus. ANAL. SCI. 39, 901–909 (2023). https://doi.org/10.1007/s44211-023-00295-8

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