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Observation of the phase-separation multiphase flow using a polyethylene glycol/phosphate mixed solutions and the aqueous two-phase distribution of red blood cells in the flow system

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Abstract

Phase-separation multiphase flow at a liquid–liquid interface was successfully formed in an aqueous two-phase system of polyethylene glycol/phosphate mixed solutions when fed into a microchannel (100 µm wide and 40 µm deep) on a microchip and a fused-silica capillary tube (100 µm ID). As one example, tube radial distribution flow (annular flow) was observed when 10.0 wt% polyethylene glycol 6000 and 8.5 wt% dipotassium hydrogen phosphate aqueous solution containing 1.0 mM Rhodamine B was fed at 40 ℃, recorded by bright field microscopy. It exhibited a dipotassium hydrogen phosphate-rich inner phase and polyethylene glycol-rich outer phase. Effects of conditions including composition, flow rate, viscosity, and contact angle on tube radial distribution flow were analyzed. It was found out that although the viscosity of PEG-rich solution was much higher than that of phosphate-rich one, the phase configuration in tube radial distribution flow did not necessarily obey the viscous dissipation law in untreated microchannel and capillary tube, as well as for all the types of PEG/phosphate mixed solution the PEG-rich solution occupied the outer phase near the ODS-treated inner wall of both microchannel and capillary tube against the law. To assess the use of microfluidic flow in applications, we examined the distribution of red blood cells in the inner and outer phases fed into double capillary tubes with different inner diameters. Cell distribution was found to concentrate in the inner (dipotassium hydrogen phosphate-rich) phase compared to the outer (polyethylene glycol-rich) phase at a ratio of 1.8.

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Data Availability Statement

The microflow system gave a useful clue to develop the separation and extraction of cells and biomolecules in a microspace.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (MEXT) (No. 17H03083).

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Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto, 610-0321, Japan

    Kazushi Nishimura, Chihiro Matsushita & Kazuhiko Tsukagoshi

  2. Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku-Machi, Tosu, Saga, 841-0052, Japan

    Kenichi Yamashita

  3. Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higasi-ku, Fukuoka, 812-8582, Japan

    Masaharu Murata

Authors
  1. Kazushi Nishimura
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  2. Chihiro Matsushita
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  5. Kazuhiko Tsukagoshi
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Correspondence to Kazuhiko Tsukagoshi.

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Nishimura, K., Matsushita, C., Yamashita, K. et al. Observation of the phase-separation multiphase flow using a polyethylene glycol/phosphate mixed solutions and the aqueous two-phase distribution of red blood cells in the flow system. ANAL. SCI. 39, 537–546 (2023). https://doi.org/10.1007/s44211-022-00259-4

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  • DOI: https://doi.org/10.1007/s44211-022-00259-4

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