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Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor

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Abstract

Animals sense odorants using olfactory receptors. Many trials have been conducted to develop artificial odorant sensors using olfactory receptors. However, the development has been hindered by the difficulty in obtaining olfactory receptors. In this study, we expressed an olfactory receptor, cOR52, using a wheat germ cell-free synthesis system. The functionality of the expressed cOR52 was confirmed by ligand concentration-dependent interactions with the mini-G protein. The expressed cOR52 was immobilized on a graphene field-effect transistor. The cOR52-modified graphene field-effect transistor exhibited a ligand-specific response between 100 nM and 100 µM. This approach seems to be applicable for other olfactory receptors. Therefore, it will be possible to develop an odorant sensor equipped with various olfactory receptors by this method.

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All data generated or analyzed during this study are included in this published article and its supplementary information file.

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Acknowledgements

The authors would like to thank Prof. Daisuke Yoshino of Tokyo University of Agriculture and Technology for the experimental supports of the fluorescence microscopic images. This work was supported by grants from JSPS-KAKENHI (18K14060, 20K15745, 20H02159, 20H02532 and 21H01336) and JST (ACT-X Grant Number JPMJAX201C), Japan.

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Author notes

  1. Tomoya Yoshii, Ikumi Takayama, Yosuke Fukutani and Takashi Ikuta contributed equally to this work.

Authors and Affiliations

  1. Department of Advanced Applied Physics, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Tomoya Yoshii, Takashi Ikuta & Kenzo Maehashi

  2. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Ikumi Takayama, Yosuke Fukutani & Masafumi Yohda

Authors
  1. Tomoya Yoshii
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  2. Ikumi Takayama
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  3. Yosuke Fukutani
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  4. Takashi Ikuta
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  5. Kenzo Maehashi
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  6. Masafumi Yohda
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Corresponding author

Correspondence to Masafumi Yohda.

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Yoshii, T., Takayama, I., Fukutani, Y. et al. Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor. ANAL. SCI. 38, 241–245 (2022). https://doi.org/10.1007/s44211-022-00073-y

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  • DOI: https://doi.org/10.1007/s44211-022-00073-y

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