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UV-B-induced modulation of constitutive heterochromatin content in Arabidopsis thaliana

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Abstract

Sunlight regulates transcriptional programs and triggers the shaping of the genome throughout plant development. Among the different sunlight wavelengths that reach the surface of the Earth, UV-B (280–315 nm) controls the expression of hundreds of genes for the photomorphogenic responses and also induces the formation of photodamage that interfere with genome integrity and transcriptional programs. The combination of cytogenetics and deep-learning-based analyses allowed determining the location of UV-B-induced photoproducts and quantifying the effects of UV-B irradiation on constitutive heterochromatin content in different Arabidopsis natural variants acclimated to various UV-B regimes. We identified that UV-B-induced photolesions are enriched within chromocenters. Furthermore, we uncovered that UV-B irradiation promotes constitutive heterochromatin dynamics that differs among the Arabidopsis ecotypes having divergent heterochromatin contents. Finally, we identified that the proper restoration of the chromocenter shape, upon DNA repair, relies on the UV-B photoreceptor, UV RESISTANCE LOCUS 8 (UVR8). These findings shed the light on the effect of UV-B exposure and perception in the modulation of constitutive heterochromatin content in Arabidopsis thaliana.

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Acknowledgements

We are grateful to Prof. Roman Ulm for providing the uvr8-6 seeds. This research was funded by a grant from the French National Research Agency (ANR-20-CE20-002) and supported by the EPIPLANT Groupement de Recherche (CNRS, France). K.G. was supported by the ERASMUS program for higher education.

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  1. Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67000, Strasbourg, France

    Philippe Johann to Berens, Kinga Golebiewska, Jackson Peter, Sébastien Staerck & Jean Molinier

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Johann to Berens, P., Golebiewska, K., Peter, J. et al. UV-B-induced modulation of constitutive heterochromatin content in Arabidopsis thaliana. Photochem Photobiol Sci 22, 2153–2166 (2023). https://doi.org/10.1007/s43630-023-00438-w

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