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Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

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Abstract

Photodynamic inactivation (PDI) has received increasing attention as a promising approach to combat Candida albicans infections. This study aimed to evaluate the synergistic effect of a new BODIPY (4,4-difluoro-boradiazaindacene) derivative and hydrogen peroxide on C. albicans. BDP-4L in combination with H2O2 demonstrated enhanced photokilling efficacy. In suspended cultures of C. albicans, the maximum decrease was 6.20 log and 2.56 log for PDI using BDP-4L (2.5 μM) with or without H2O2, respectively. For mature C. albicans biofilms, 20 μM BDP-4L plus H2O2 eradicated C. albicans, causing an over 6.7 log count reduction in biofilm-associated cells, while only a reduction of ~ 1 log count was observed when H2O2 was omitted. Scanning electron microscopy analysis and LIVE/DEAD assays suggested that PDI using BDP-4L plus H2O2 induced more damage to the cell membrane. Correspondingly, amplification of nucleic acids release was observed in biofilms treated with the combined PDI. Additionally, we also discovered that the addition of hydrogen peroxide potentiated the generation of 1O2 in PDI using the singlet oxygen sensor green probe. Collectively, BDP-4L combined with H2O2 presents a promising approach in the treatment of C. albicans infections.

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Data availability statement

The data presented in this study are available on request from the corresponding author.

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Acknowledgements

The research was financially supported by the National Natural Science Foundation of China (No. 82030113) and Shanghai Municipal Science & Technology Pillar Program for Bio-pharmaceuticals (21S11907200).

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Authors and Affiliations

  1. School of Pharmacy, Fudan University, No.826, Rd. Zhangheng, Shanghai, 200000, China

    Mengran Wang, Miyang Wan, Lu Gan, Jingtao Chen, Weili Zhao & Jiyang Li

  2. Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Science, No.150, Rd. Fucheng, Hangzhou, 310000, China

    Kedan Gu

  3. Department of Stomatology, Huashan Hospital, Fudan University, No.12, Rd. Wulumuqi, Shanghai, 200000, China

    Hang Shi

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  1. Mengran Wang
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Correspondence to Weili Zhao, Hang Shi or Jiyang Li.

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Wang, M., Gu, K., Wan, M. et al. Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative. Photochem Photobiol Sci 22, 1695–1706 (2023). https://doi.org/10.1007/s43630-023-00408-2

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