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Time-resolved diffusion reveals photoreactions of BLUF proteins with similar functional domains

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Abstract

BLUF (blue light sensor using flavin) proteins are the blue light receptors that consist of flavin-binding BLUF domains and functional domains. Upon blue light excitation, the hydrogen bond network around the flavin chromophore changes, and the absorption spectrum in the visible region shifts to red. Light signal received in the BLUF domain is intramolecularly or intermolecularly transmitted to the functional region. In this review, the reactions of three BLUF proteins with similar EAL functional groups within the protein (BlrP1, and YcgF), or with a separated target protein (PapB) are described using time-resolved diffusion technique. The diffusion coefficients (D) of the BLUF domains did not significantly change upon photoexcitation, whereas those of the full-length proteins BlrP1 and YcgF and the PapB–PapA system significantly decreased. The changes in D should be due to diffusion-sensitive conformational changes (DSCC) that alter the friction of diffusion. The time constants of the major D changes of BlrP1 and PapB–PapA were similar (~ 20 ms), although the magnitude of the friction change depended on the proteins. Similarities and differences among the reactions of these proteins were clarified from the viewpoint of DSCC.

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Acknowledgements

The authors acknowledge all authors who have contributed to the papers cited in this review. This work was supported by a Grant-in-aid for Scientific Research on Innovative Areas (research in a proposed research area) (Nos. JP20107003, and JP25102004) and a Grant-in-aid for Scientific Research (17H03008, 21H01885, 21K19218 to M.T. and 18H045522, 20H04708 to Y.N.) from MEXT/JSPS.

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  1. Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan

    Yusuke Nakasone & Masahide Terazima

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  1. Yusuke Nakasone
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  2. Masahide Terazima
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Correspondence to Masahide Terazima.

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This publication is dedicated to Prof. Silvia E. Braslavsky, a pioneer in photobiology and photobiophysics, on the occasion of her 80th birthday.

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Nakasone, Y., Terazima, M. Time-resolved diffusion reveals photoreactions of BLUF proteins with similar functional domains. Photochem Photobiol Sci 21, 493–507 (2022). https://doi.org/10.1007/s43630-022-00214-2

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