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Investigation on the in vitro anti-Trichophyton activity of photosensitizers

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Abstract

Onychomycosis is the most common disease caused by fungal nail infections, and often caused by dermatophytes. This infection is very resistant to antifungal treatments, and promising Photodynamic Therapy (PDT) mediated treatments has been presented as a multitarget tracking. Optimization of PDT guide for uptake time, concentration of photosensitizers (PS) and the light dose to inactivate Trichophyton mentagrophytes. Curcumin derivatives, porphyrin Chlorin e6 (CHL-E6) and Chlorin-P6-6-N-butylamide-7-methyl-ester (CHL-butyl) were evaluated. PS photobleaching was observed on the hyphae photosensitized over the time, correlating the PS concentration and light dose of antifungal PDT. Porphyrin, Curcumin, Chl-e6 and Chl-butyl concentrations of 2.5 µg/mL, 0.025 µg/mL, 10 µg/mL and 5 µg/mL respectively, under illumination of 10.5 J/cm2 were the best antifungal conditions found in the study. Curcumin, in low concentrations, and chlorin were the PSs with higher activity anti-T. mentagrophytes.

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Acknowledgements

The authors acknowledge the financial support provided by the São Paulo Research Foundation (FAPESP/CEPOF), Proc. Nº 13/07276-1 and 2011/19720-8 (FAPESP). The "National Counsel of Technological and Scientific Development" (CNPq/INCT Proc. Nº 573587/2008-6). The “Brazilian Innovation Agency” (FINEP Proc. Nº 01.09.0241.00. APS would like to thank the CNPq grants for her Scholarship received between 2010 and 2011 Proc. Nº 381132/2010-2. Blanco K. C. thanks FAPESP for Post-doc grant 2019/13569-8 and 2021/09952-0.

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Authors and Affiliations

  1. São Carlos Institute of Physics, University of São Paulo, São Carlos Trabalhador São-carlense Av., 400, São Carlos, São Paulo, 13566-590, Brazil

    Ana Paula da Silva, Francisco Eduardo Gontijo Guimarães, Kate Cristina Blanco, Vanderlei Salvador Bagnato & Natalia Mayumi Inada

  2. Federal University for Latin American Integration, CEP 85866-000, Caixa Postal 2044, Foz do Iguaçu, PR, Brazil

    Marciana Pierina Uliana

  3. Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo, 13565-905, Brazil

    Kleber Thiago de Oliveira

  4. Texas A & University, College Station, TX, USA

    Kate Cristina Blanco & Vanderlei Salvador Bagnato

Authors
  1. Ana Paula da Silva
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  2. Marciana Pierina Uliana
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  3. Francisco Eduardo Gontijo Guimarães
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  4. Kleber Thiago de Oliveira
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  5. Kate Cristina Blanco
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  6. Vanderlei Salvador Bagnato
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  7. Natalia Mayumi Inada
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Correspondence to Kate Cristina Blanco.

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da Silva, A.P., Uliana, M.P., Guimarães, F.E.G. et al. Investigation on the in vitro anti-Trichophyton activity of photosensitizers. Photochem Photobiol Sci 21, 1185–1192 (2022). https://doi.org/10.1007/s43630-022-00205-3

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  • DOI: https://doi.org/10.1007/s43630-022-00205-3

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