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Effect of Locally Administered Denosumab on Bone Graft Healing in Rabbit Critical-Size Calvarial Defects.

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Abstract

Background/Aim

Denosumab is a human monoclonal immunoglobulin G2 antibody developed from the ovarian cells of Chinese hamsters. We aimed to histomorphometrically and radiologically evaluate the effects of xenografts used with local denosumab on the healing of defect sites using rabbit skulls.

Materials and Methods

Two 10-mm diameter critical-size defects were created in 16 rabbits. The defect areas were filled with xenografts and xenograft + 3 mg denosumab in the control and denosumab groups (DEN), respectively. We evaluated new bone, residual graft, soft tissue areas, and bone volume in 4- and 8-week study groups.

Results

Histomorphometrically, there were no statistically significant differences between groups at both 4 and 8 weeks regarding residual graft, new bone, and soft tissue area (p > 0.05). The 4-week residual graft control group values were significantly higher than the 8-week values (p < 0.05). The soft tissue area was significantly greater in the 4-week compared with the 8-week DEN group (p < 0.05). The radiologically measured total bone volume was significantly greater in the 8-week specimens than in the 4-week specimens (p < 0.05).

Conclusion

In this study, denosumab used locally with bone grafts, showed no direct effect on new and total bone volume.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hacettepe University, Ankara, Turkey

    Taha Özer, Özgür Başlarlı & Alper Aktaş

  2. Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey

    Emre Barış

  3. Vocational School of Health, Ankara University, Ankara, Turkey

    Mert Ocak

Authors
  1. Taha Özer
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  2. Özgür Başlarlı
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  3. Alper Aktaş
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  4. Emre Barış
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  5. Mert Ocak
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Contributions

Author Contribution

Conceptualization: Taha Özer, Özgür Başlarlı, Alper Aktaş; Methodology: Taha Özer, Emre Barış; Formal analysis and investigation: Taha Özer, Emre Barış, Mert Ocak; Writing - original draft preparation: Taha Özer; Writing - review and editing: Alper Aktaş; Funding acquisition: Taha Özer, Özgür Başlarlı; Resources: Emre Barış, Mert Ocak; Supervision: Alper AktaşAll authors read and approved the final manuscript.

Corresponding author

Correspondence to Taha Özer.

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Conflict Of Interest

The authors have no competing interests to declare that are relevant to the content of this article.

Ethical Approval

This study protocol was performed in accordance with National Institutes of Health ARRIVE guidelines for the care and use of laboratory animals and independently reviewed and approved by the Animal Experiments Local Ethics Committee of Hacettepe University on 29 May 2018 under the identification number, 2017/47-03.

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Özer, T., Başlarlı, Ö., Aktaş, A. et al. Effect of Locally Administered Denosumab on Bone Graft Healing in Rabbit Critical-Size Calvarial Defects.. JOIO 56, 1424–1430 (2022). https://doi.org/10.1007/s43465-022-00673-w

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  • DOI: https://doi.org/10.1007/s43465-022-00673-w

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