Abstract
Fatty acid binding protein 4 is predominantly expressed in adipose tissue and functions as an important mediator of inflammation. However, small molecules that regulate its expression in adipocytes have not been adequately characterized. In this study, we found that trilobatin dose-dependently attenuates the mRNA expression and protein levels of fatty acid binding protein 4 induced by lipopolysaccharide in the differentiated 3T3-L1 adipocytes. Meanwhile, trilobatin also reduced the mRNA and protein levels of fatty acid binding protein 4 in the epididymal white adipose tissue and the contents of pro-inflammatory cytokines, including tumor necrosis factor α and interleukin-6 in the serum of obese-hyperglycemic mice. In addition, trilobatin protected against the decrease of microRNA precursors, including premiR-375, premiR-338, and premiR-129, but only miR-375 inhibitor prohibited the role of trilobatin on the expression of fatty acid binding protein 4, suggesting that miR-375 might be involved in trilobatin regulating this protein expression in LPS-treated differentiated 3T3-L1 adipocytes. All these findings suggest that trilobatin might be a promising compound for the management of obesity due to its anti-inflammatory activity by suppressing the expression of fatty acid binding protein 4 in adipocytes.
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The data and materials of the current study are available from the corresponding author on reasonable request.
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Funding
This work was supported by the Scientific and Technological Research Program of Chongqing Municipal Education Commission (Grant, KJZD-K202101102, KJQN201901139) and Chongqing Science & Technology Bureau (cstc2019jcyj-msxmX0427).
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LXG and WYC performed research and analyzed results. YF performed statistical analysis. LJH, YF, and YL wrote and edited the manuscript. LJH and YL designed the project and contributed the research questions. All authors have read the final version of this paper and approved its publication.
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Li, X., Wu, Y., Jiang, X. et al. Trilobatin Ameliorates Inflammatory Response by Regulating the miR375-Mediated Fatty Acid Binding Protein 4 Expression in Adipocytes. Rev. Bras. Farmacogn. 33, 214–221 (2023). https://doi.org/10.1007/s43450-022-00356-5
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DOI: https://doi.org/10.1007/s43450-022-00356-5