Abstract
Marrubium astracanicum Jacq., Lamiaceae, is an herbaceous plant distributed in Iran, Turkey, and southern Caucasia. In the present study, α-glucosidase inhibitory principles of M. astracanicum aerial parts were investigated by a bioassay-guided approach. The extraction procedure from the plant aerial parts was carried out successively using petroleum ether, ethyl acetate, and methanol. The ethyl acetate extract, with the highest in vitro α-glucosidase inhibitory activity, was subjected to further chemical investigation. The structures of the isolated compounds were elucidated by 1H-NMR and 13C-NMR spectral analyses as apigenin-7-O-(3′′-(E)-p-coumaroyl)-β-d-glucoside, apigenin-7-O-(6′′-(E)-p-coumaroyl)-β-d-glucoside, martynoside, apigenin-7-O-β-d-glucoside, and verbascoside. In addition, the antidiabetic potential of the isolated compounds was evaluated in an in vitro α-glucosidase inhibitory assay, and kinetic and molecular docking studies were done for the most active compound. Apigenin-7-O-(3′′-(E)-p-coumaroyl)-β-d-glucoside and apigenin-7-O-(6′′-(E)-p-coumaroyl)-β-d-glucoside showed the most potent in vitro α-glucosidase inhibitory activity with IC50 values of 57.4 ± 1.2 and 186.4 ± 2.5 μM, respectively, which was about 4 to 13 times lower than the IC50 value of the reference compound acarbose (751.2 ± 0.4 μM).
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This research was supported by Tehran University of Medical Sciences and Health Services Grants (No. 99-1-104-48267).
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RK and AH carried out the phytochemical analysis. MRD and MK supervised this study and suggested the original idea. MRD also has participated in interpreting related spectra and writing the manuscript. MAF and SM have contributed to enzyme inhibition assay. HPK conducted the molecular docking study. The current manuscript was critically read and approved by all authors.
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Kazemi, R., Delnavazi, MR., Parsa-Khankandi, H. et al. α-Glucosidase Inhibitors from Marrubium astracanicum: Isolation and Molecular Docking. Rev. Bras. Farmacogn. 32, 618–626 (2022). https://doi.org/10.1007/s43450-022-00287-1
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DOI: https://doi.org/10.1007/s43450-022-00287-1