Abstract
Chemical analysis of the aqueous and n-butanol fractions of methanolic extract of Stipagrostis plumosa Munro ex T.Anderson, Poaceae, aerial parts led to isolation of seven known phenolic glycosides 3,4′-dihydroxypropiophenone-3-O-β-d-glucoside, 5′-methoxy-3,4′-dihydroxypropiophenone-3-O-β-d-glucoside, 3′5′-dimethoxy-3,4′-dihydroxypropiophenone-3-O-β-d-glucoside, 3,4,5-trimethoxyphenyl-β-d-glucopyranose, tricin-7-O-rutinoside, tricin-7-O-β-d-glucoside, and apigenin-6-C-α-l-arabinopyranosyl-8-C-β-d-glucopyranoside. The cytotoxic activity of the isolates, expressed as IC50, was assessed against five different tumor cell lines (JEG-3, HeLa, MCF-7, NCI-H295R, and Hep-G2), cancer like cells (MCF-10A), and normal cells (HDFa) using tamoxifen as a reference drug. All tested compounds exhibited promising activity against JEG-3 cell line as compared to the reference tamoxifen. Fluorescence imaging study indicated the disruption of cell nucleus and lower integrity of F-actin filament, suggesting a potential two-phase cytotoxicity mechanism.
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MSAA: manuscript submission; MAAO and MSAA: isolation of compounds and interpretation of spectroscopic data; MY and MB: cytotoxicity assays; and MAAO, MSAA, MY, and MB: writing and revising the manuscript. All authors have contributed to the writing of the manuscript and approved the final submission.
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Bawazeer, M., Orabi, M.A.A., Yaseen, M. et al. Cytotoxic Potential of Phenolic Glycosides from Stipagrostis plumosa. Rev. Bras. Farmacogn. 31, 842–847 (2021). https://doi.org/10.1007/s43450-021-00206-w
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DOI: https://doi.org/10.1007/s43450-021-00206-w