Coptis chinensis Franch., Ranunculaceae, is a widely used herbal medicine in Asian countries. It exhibits many important pharmacological effects. The rhizome is used as the crude drug for this medicinal plant. The objective of this study was to determine whether the aerial part (stem and leaves) can be employed as a complementary material for medicinal purposes. Ultraperformance liquid chromatography coupled to a hybrid quadrupole-orbitrap high-resolution mass spectrometry analysis was applied to detect fifteen alkaloids. Network pharmacology analysis indicated that these compounds might interact with important glucose-lipid metabolism–related targets. Appropriate dose of streptozotocin and high-fat diet were used to establish the model of glucose-lipid disorder in Kunming mice. Blood glucose level was determined by handheld blood glucose meter. Peroxidase antiperoxidase kit was used to test the serum total cholesterol and triacylglyceride level. Hematoxylin/eosin staining was performed to analyze the degree of damage in the liver and kidney. Our data demonstrated that aerial part contained a similar chemical composition with the crude drug. Network pharmacology analysis and animal experiments also indicated that extracts from the aerial parts exhibited a good glucose-lipid metabolism regulatory activity.
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All authors are grateful to their affiliation institutions which provided research conditions and access to analytical instrumentations.
Financial support was supported by the Department of Science and Technology of Sichuan Province (2020YFS0498) and the Chengdu University of Traditional Chinese Medicine Key Laboratory of Systematic Research of Distinctive Chinese Medicine Resources in Southwest China (2020JCRC015).
All experiments were performed consistent with animal use followed by Chengdu University of Traditional Chinese Medicine of Laboratory Animal Ethics Committee. Ethical approval number: 2020-05.
Conflict of Interest
The authors declare that they have no conflicts of interest.
Animal Ethical Approval
All experiments were performed in compliance with international rules on the care and use of laboratory animals. The study was approved by the Chengdu University of Traditional Chinese Medicine Laboratory Animal Ethics Committee. Ethical protocol number: 2020-05.
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Lou, G., Xiong, H., Gan, Q. et al. UPLC-Q-Orbitrap HRMS Analysis of Coptis chinensis Aerial Parts and Its Regulatory Activity on Glucose-lipid Metabolism. Rev. Bras. Farmacogn. 31, 24–31 (2021). https://doi.org/10.1007/s43450-020-00124-3
- Coptidis rhizoma
- Chinese goldthread
- Isoquinoline alkaloid
- Metabolic disorder
- Protoberberine alkaloid