Abstract
Dipteryx lacunifera Ducke, Fabaceae, popularly known as “fava-de-morcego” and “garampara,” comprises pleasant tasting kernels and is commonly consumed by inhabitants northeast of Brazil. In the present study, among six flavonoids, two are reported for the first time as natural products, 6,3′,4′-trihydroxyflavone (2) and 4-O-metilsulfuretin (4); two reported for the first time in the species isoliquiritigenin (1) and homobutein (6); and two already known flavonoids, 7,3′,4′-trihydroxyflavone (3) and (−)-butin (5), were isolated from fruits and kernels of D. lacunifera. The compounds were isolated by chromatographic techniques and elucidated by spectrometric data (1D and 2D NMR and mass). Compounds 1–3 and 5 showed interesting cytotoxicity against tumor cell lines with inhibitory concentration (IC50) values ranging from 21.13 to 2.47 μg/ml. In addition, compounds 1 and 2 showed antimalarial activity against P. falciparum with 81.25 and 88.98% of parasitemia reduction, respectively.
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Acknowledgments
We are grateful to Prof. Norberto Peporine Lopes (USP-RP) for HRMS analysis.
Funding
The authors received financial support from the Brazilian agency Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and National Institute of Science and Technology (INCT BioNat, grant #465637/2014-0).
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LSA (MSc student), MSO (Undergraduate student) and GMVJ contributed in isolation and identification of the compounds. RWRS, DD and PMPF contributed with cytotoxic activity of compounds 3 and 5. PMPF and CP contributed with cytotoxic activity of compounds 1 and 2. FPV, BAMS and FFB contributed with antimalarial activity and hemolysis. LSA, DD and FPV also contributed with the draft of the article. GMVJ contributed with revision and critical reading of the manuscript. GMVJ and MHC has coordinated and supervised the laboratory work. All the authors have read the final manuscript and approved the submission.
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Alexandre, L.S., Oliveira, M.S., Dittz, D. et al. Flavonoids, Cytotoxic, and Antimalarial Activities of Dipteryx lacunifera. Rev. Bras. Farmacogn. 30, 544–550 (2020). https://doi.org/10.1007/s43450-020-00082-w
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DOI: https://doi.org/10.1007/s43450-020-00082-w