Abstract
This study was designed to investigate the anti-inflammatory, analgesic activity of azadirone from Toona sinensis (Juss.) M. Roem., Meliaceae, and explore its potential mechanisms. Anti-inflammatory activities of azadirone were evaluated using dimethylbenzene-induced ear edema, acetic acid–induced vascular permeability, and cotton ball granuloma in mice; in addition, its analgesic activities were investigated using acetic acid writhing, hot plate pain, and formalin pain tests. The results showed that azadirone had obvious anti-inflammatory and analgesic effects in animals. Further studies revealed that azadirone can reduce levels of TNF-α, IL-6, and IL-1 in lipopolysaccharide-stimulated RAW 264.7 cells. In addition, azadirone can downregulate COX-2 and substance P in spinal cord tissues of formalin-stimulated mice. Also, expressions of ERK, P38, and JNK in RAW 264.7 cells were suppressed by azadirone. Collectively, azadirone has promising anti-inflammatory and analgesic potentials, and its mechanism is closely related to the reduction of pro-inflammatory cytokines and the blocking of MAPK signal pathway.
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HLZ contributed in the collection of T. sinensis samples, compound preparation, running laboratory works, analysis of data, and writing manuscript. MZL contributed in running laboratory works, compound preparation, and analysis of data; designed the studies; reviewed data; wrote and reviewed manuscript; designed and discussed the studies.
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Li, M., Zhang, H. Azadirone from Toona sinensis possesses anti-inflammatory and analgesic activities via suppressing MAPK signal pathway. Rev. Bras. Farmacogn. 30, 494–502 (2020). https://doi.org/10.1007/s43450-020-00063-z
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DOI: https://doi.org/10.1007/s43450-020-00063-z