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Cisplatin effect on digital cytomorphometric and bioinformatic tumor cell characteristics in rat ovarian cancer model–a preliminary study

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Abstract

Background

Ovarian cancer is one of the most common diseases of the female reproductive system. The aim of this study was the investigation of the antitumor cisplatin effect on ascitic fluid tumor cells in the ovarian cancer experimental model by digital cytomorphometry and cell bioinformatic analysis.

Methods

Female Wistar rats were inoculated by ovarian cancer strain. After ovarian cancer transplantation rats were divided into two groups: control group—without cisplatin treatment, the experimental group—under cisplatin treatment. The ascitic fluid was taken on the 9-th day after tumor cell inoculation. Digital cytomorphometric and cytobioinformatic analysis were used to study ascitic fluid cancer cell morphofunctional changes under cisplatin treatment.

Results

Digital cytomorphometric characteristics of rat ovarian cancer ascitic cells were obtained. Tumor cells were classified into four groups according to their geometric size: small, medium, large, “gigantic”. The algorithm and the computer program based on tumor cell morphometric characteristics were created to calculate such cell bioinformatic characteristic as information redundancy coefficient R. Three parameters were determined as the criteria of cisplatin effect on cancer cells: cell number, nuclear/cytoplasmic ratio, R-value.

Conclusions

Data obtained suggest that cisplatin reduces the total cell number, the nuclear/cytoplasmic ratio and R-value thus indicates a decrease in cellular resistance and adaptive potential. The digital cytomorphometry and bioinformatics could be recommended as a testing system in the experimental or clinical study.

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Acknowledgements

This work was partially financially supported by the Ministry of Science and Higher Education of the Russian Federation, Grant RFMEFI58117X0020.

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Authors and Affiliations

  1. Department of Cancer Chemoprevention and Oncopharmacology, N. N. Petrov National Medical Research Center of Oncology, 68, Leningradskaya Ul., Pesochnyi, St. Petersburg, 197758, Russia

    Nadezhda T. Zhilinskaya, Vladimir G. Bespalov, Alexander L. Semenov, Elena D. Ermakova, Grigory V. Tochilnikov & Valerii A. Alexandrov

  2. International Research Centre “Biotechnologies of the Third Millennium”, ITMO University, 49, Kronversky Pr, St. Petersburg, 197101, Russia

    Vladimir G. Bespalov, Nadezhda V. Barakova & Denis A. Baranenko

  3. Graduate School of Biotechnologies and Food Science, Institute of Biomedical Systems and Biotechnologies, Peter the Great St. Petersburg Polytechnic University, 29, Polytekhnicheskaya, St. Petersburg, 195251, Russia

    Nadezhda T. Zhilinskaya & Elena D. Ermakova

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  1. Nadezhda T. Zhilinskaya
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  2. Vladimir G. Bespalov
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  3. Alexander L. Semenov
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  4. Elena D. Ermakova
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  5. Grigory V. Tochilnikov
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  6. Nadezhda V. Barakova
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  7. Valerii A. Alexandrov
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  8. Denis A. Baranenko
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Contributions

Conception and design of the work: Vladimir Bespalov. Acquisition: Alexander Semenov. Analysis: Grigory Tochilnikov, Elena Ermakova. Interpretation of data for the work: Nadezhda Zhilinskaya, Nadezhda Barakova. Drafting the work: Valerii Alexandrov. Revising for important intellectual content: Denis Baranenko. Final approval of the version to be published: Nadezhda Zhilinskaya.

Corresponding author

Correspondence to Nadezhda T. Zhilinskaya.

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Authors declared that they have no conflict of interest.

Statement of human and animal rights

This article does not contain any studies with human participants performed by any of the authors. Maintenance and care of all animals were carried out according to the ethical principles established by the European Convention for the protection of vertebrate animals, used for experimental and other scientific purposes (accepted in Strasbourg 18.03.1986 and confirmed in Strasbourg 15.06.2006), and approved by Local ethical committee of the N.N. Petrov National Medical Research Center of Oncology.

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Zhilinskaya, N.T., Bespalov, V.G., Semenov, A.L. et al. Cisplatin effect on digital cytomorphometric and bioinformatic tumor cell characteristics in rat ovarian cancer model–a preliminary study. Pharmacol. Rep 73, 642–649 (2021). https://doi.org/10.1007/s43440-020-00199-8

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  • DOI: https://doi.org/10.1007/s43440-020-00199-8

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