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Lycopene increases metabolic activity of rat liver CYP2B, CYP2D and CYP3A

Pharmacological Reports volume 72, pages 156–165 (2020)Cite this article

Abstract

Background

Lycopene as a naturally occurring carotenoid is a common part of the human diet. Several beneficial properties of lycopene have been identified, with the most studied being anti-cancer and antioxidant activity. However, no evidence of possible drug–drug or drug–food supplement interactions has been found.

Methods

We studied the in vivo effect of lycopene on the selected rat liver cytochromes P450 (CYPs): CYP1A2, CYP2B, CYP2C11, CYP2C6, CYP2D, and CYP3A. Lycopene was administered to rats intragastrically at doses of 4, 20, and 100 mg/kg/day for 10 consecutive days. Total protein content, P450 Content, and metabolic activity of selected CYPs were evaluated in the rat liver microsomal fraction.

Results

Increased CYP2B, CYP2D, and CYP3A metabolic activities were observed in animals treated with the lycopene dose of 100 mg/kg/day. The content of CYP3A1 protein was increased by the dose of 100 mg/kg/day and CYP3A2 protein was increased by all administered doses of lycopene.

Conclusion

The results of our study indicate that lycopene increased the metabolic activity of enzymes that are orthologues to the most clinically important human enzymes involved in xenobiotic metabolism. The risk of pharmacokinetic interactions between lycopene dietary supplements and co-administered drugs should be evaluated.

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Acknowledgements

This publication was written with the support of the Specific University Research grant (MUNI/A/1550/2018) provided by the Ministry of Education, Youth and Sports of the Czech Republic and by the Specific University Research Grant (MUNI/A/0976/2018) and by the funds from the Faculty of Medicine, Masaryk University junior researcher Ondrej Pes.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

    Kristýna Nosková, Ondřej Zendulka, Markéta Strakošová & Jan Juřica

  2. RECETOX Centre, Masaryk University, Brno, Czech Republic

    Gabriela Dovrtělová

  3. Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno, Czech Republic

    Ondřej Peš

  4. Masaryk Memorial Cancer Institute, Brno, Czech Republic

    Jan Juřica

  5. Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic

    Jan Juřica

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  1. Kristýna Nosková
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  2. Gabriela Dovrtělová
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Correspondence to Jan Juřica.

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Nosková, K., Dovrtělová, G., Zendulka, O. et al. Lycopene increases metabolic activity of rat liver CYP2B, CYP2D and CYP3A. Pharmacol. Rep 72, 156–165 (2020). https://doi.org/10.1007/s43440-019-00007-y

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  • DOI: https://doi.org/10.1007/s43440-019-00007-y

Keywords

  • Lycopene
  • Rat
  • Cytochrome P450
  • CYP3A
  • Induction