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Sonoprocessing coupled to spray drying as a novel strategy to encapsulate bioactive compounds from acerola pomace extract into Saccharomyces cerevisiae cells

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Abstract

This study evaluates the encapsulation of phytoactives from acerola (Malpighia emarginata) pomace extract (APE) into Saccharomyces cerevisiae cells via sonoprocessing coupled to spray drying (SPSD process). The effect of acoustic energy density (AED; 0–333.3 W/L), APE pH (3–11), and spray drying inlet temperature (T; 150–190 °C) on the encapsulation efficiency (EE) and yield (EY) of phenolic compounds and anthocyanins were investigated through a Box–Behnken experimental design (BBD). Results demonstrated that maximized EEs (> 63%) and EYs (> 126.7 mg/100 g) were obtained using the highest AED (333.3 W/L) and alkaline APE (pH 11), while the optimal T was set at 170 °C. Likewise, these process conditions also enhanced the antioxidant activity of biocapsules which positively correlated with the concentration of yeast encapsulated phytoactives by SPSD. Overall, our results demonstrate the versatility of SPSD protocol for manufacturing bioactive-enriched yeast biocapsules using complex mixtures of phytoactives. Further, it presents an innovative and sustainable roadmap for the management and repurposing of agri-food byproducts such as acerola pomace and other secondary streams of the food industry.

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All data generated or analyzed during this study are included in this manuscript.

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Acknowledgements

The authors are grateful to the Universidade Federal do Rio Grande do Norte (UFRN) and the Chemical Engineering Department (DEQ/UFRN) for technical support.

Funding

EWVA was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Grant number 140208/2022-4.

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Authors and Affiliations

  1. Laboratory of Food Engineering, Chemical Engineering Department, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Natal, RN, 59078-970, Brazil

    Eduardo Wagner Vasconcelos de Andrade, Roberta Targino Hoskin & Márcia Regina da Silva Pedrini

  2. Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, USA

    Roberta Targino Hoskin

  3. UMR PAM 1517, Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, 21000, Dijon, France

    Sébastien Dupont & Laurent Beney

  4. Farmaceutical Science Department, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil

    Thiago Caon

Authors
  1. Eduardo Wagner Vasconcelos de Andrade
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  2. Roberta Targino Hoskin
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  3. Sébastien Dupont
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  4. Laurent Beney
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  6. Márcia Regina da Silva Pedrini
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Contributions

EWVA: conceptualization, methodology, investigation, formal analysis, writing (draft and review), and visualization. SD, LB, and TC: writing (review). RTH and MRSP: methodology, resources, supervision, and writing (draft and review). All authors read and approved the manuscript.

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Correspondence to Márcia Regina da Silva Pedrini.

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de Andrade, E.W.V., Hoskin, R.T., Dupont, S. et al. Sonoprocessing coupled to spray drying as a novel strategy to encapsulate bioactive compounds from acerola pomace extract into Saccharomyces cerevisiae cells. Syst Microbiol and Biomanuf (2024). https://doi.org/10.1007/s43393-024-00248-w

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