Abstract
In the environment, aquatic organisms are not only directly exposed to pollutants, but the effects can be exacerbated along the food chain. In this study, we investigated the effect of the food (water flea) on the secondary consumer (zebrafish) with the exposure diclofenac (DCF) Both organisms were exposed to an environmentally relevant concentrations (15 µg/L) of diclofenac for five days, and zebrafish were fed exposed and non-exposed water fleas, respectively. Metabolites of the water fleas were directly analyzed using HRMAS NMR, and for zebrafish, polar metabolite were extracted and analyzed using liquid NMR. Metabolic profiling was performed and statistically significant metabolites which affected by DCF exposure were identified. There were more than 20 metabolites with variable importance (VIP) score greater than 1.0 in comparisons in fish groups, and identified metabolites differed depending on the effect of exposure and the effect of food. Specifically, exposure to DCF significantly increased alanine and decreased NAD + in zebrafish, which means energy demand was increased. Additionally, the effects of exposed food decreased in guanosine, a neuroprotective metabolite, which explained that the neurometabolic pathway was perturbated by the feeding of exposed food. Our results which short-term exposed primary consumers to pollutants indirectly affected the metabolism of secondary consumers suggest that the long-term exposure further study remains to be investigated.
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This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (Grant number NRF-2020R1I1A2075016).
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All authors contributed to the study conception and design. Material preparation, data collection was performed by Youzhen Li, analysis were performed by Youzhen Li, Seonghye Kim and Sujin Lee. The first draft of the manuscript was written by Suhkmann Kim and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Li, Y., Kim, S., Lee, S. et al. Metabolic effects of diclofenac on the aquatic food chain – 1 H-NMR study of water flea-zebrafish system. Toxicol Res. 39, 307–315 (2023). https://doi.org/10.1007/s43188-022-00167-9
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DOI: https://doi.org/10.1007/s43188-022-00167-9