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Antiphotoaging properties of Zingiber montanum essential oil isolated by solvent-free microwave extraction against ultraviolet B-irradiated human dermal fibroblasts

Abstract

Maintaining youthful skin from photoaging with natural products, including essential oils, is a vital strategy that has piqued the interest of researchers in the pharmaceutical and cosmetic industries. This research aimed to investigate the protective properties of Zingiber montanum (J. Koenig) Link ex A. Dietr. essential oil against ultraviolet B (UVB)-induced skin damage and photoaging in normal human dermal fibroblast (HDFn) cells. The essential oil was extracted from fresh plant rhizomes using solvent-free microwave extraction. Its antiphotoaging properties in HDFn cells were investigated using reactive oxygen species (ROS)-scavenging, wound healing, matrix metalloproteinases (MMP-1, MMP-3, and MMP-9) expression, procollagen synthesis, and elastase and tyrosinase inhibitory assays. The results showed that the test oil exhibited no significant toxicity in HDFn at concentrations up to 10 mg/mL, with cell viability exceeding 90%. Following UVB irradiation at 30 mJ/cm2, Z. montanum oil demonstrated time and concentration-dependent ROS radical scavenging capabilities. In a cell migration assay, the essential oil demonstrated wound-healing properties. Z. montanum oil suppressed the expression of MMPs and enhanced the synthesis of type I procollagen at a concentration of 0.1–1 mg/mL. In addition, 0.1–1 mg/mL Z. montanum oil inhibited elastase activity in a concentration-dependent manner but did not affect tyrosinase activity. From these findings, the essential oil of Z. montanum could have potential applications in developing cosmeceutical products to prevent skin photoaging.

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Abbreviations

AP-1:

Activator protein 1

GC–MS:

Gas chromatography-mass spectrometry

HDFn:

Normal human dermal fibroblast

IL:

Interleukin

MAPKs:

Mitogen-activated protein kinases

MMPs:

Matrix metalloproteinases

NAC:

N-Acetylcysteine

NF-κB:

Nuclear factor-κB

ROS:

Reactive oxygen species

UVB:

Ultraviolet B

Z. montanum :

Zingiber montanum (J. Koenig) Link ex A. Dietr

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Acknowledgments

This work was supported by the Research Fund 2018 [Grant number 12/2018] of the Research Institute, Rangsit University, Thailand, and partly supported by the Research, Development, and Engineering Fund for National Nanotechnology, National Science and Technology Development Agency (NSTDA), Thailand. This manuscript was proofread and edited by Cambridge Proofreading LLC under the support of the Research Institute of Rangsit University.

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AN funding acquisition, conceived the idea and design of the study, carried out the experimental studies and data analysis, wrote and final revised manuscript. BY participated in the conceptualization of the design, advised for statistical analysis, supervised the research project, prepared the sample extract, performed GC–MS analysis, and collected and interpreted data. RM participated in the conceptualization of the design and supervised the research project. All authors read and approved the final manuscript.

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Correspondence to Abhiruj Navabhatra.

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Navabhatra, A., Maniratanachote, R. & Yingngam, B. Antiphotoaging properties of Zingiber montanum essential oil isolated by solvent-free microwave extraction against ultraviolet B-irradiated human dermal fibroblasts. Toxicol Res. 38, 235–248 (2022). https://doi.org/10.1007/s43188-021-00107-z

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Keywords

  • Essential oil
  • Matrix metalloproteinase
  • Oxidative stress
  • Skin photoaging
  • Solvent-free microwave extraction
  • Ultraviolet B