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Orostachys japonicus ethanol extract inhibits 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice and TNF-α/IFN-γ-induced TARC expression in HaCaT cells

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Abstract

The risk of atopic dermatitis (AD)-like skin lesions has increased due to the elevated levels of allergens worldwide. Natural-origin agents, which are effective and safe, show promise for the prevention and treatment of inflammatory conditions. Orostachys japonicus (OJ) A. Berger is an ingredient of traditional herbal medicines for fever, gingivitis, and cancer in Korea, China, and Japan. However, the effect of OJ on AD-like skin lesions is unknown. Therefore, we investigated the effect of OJ ethanol extract (OJEE) on AD-like skin symptoms in mice and cells. OJEE reduced the 2,4-dinitrochlorobenzene-induced AD severity, serum levels of IgE and TARC, and mRNA levels of TARC, TNF-α, and IL-4 in NC/Nga mice. Histopathological analysis showed that OJEE reduced the thickness of the epidermis/dermis and dermal infiltration of inflammatory cells in ear tissue. Furthermore, OJEE suppressed the TNF-α/IFN-γ-increased TARC mRNA level by inhibiting NF-κB and STAT1 activation in HaCaT cells. Taken together, our findings show that OJEE reduced the risk of AD-like skin symptoms by decreasing TARC expression via inhibiting NF-κB and STAT1 activation in skin keratinocytes and thus shows promise as an alternative therapy for AD-like skin lesions.

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Acknowledgements

This work was supported by research fund of Chungnam National University

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Authors and Affiliations

  1. Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea

    Jae Ho Choi, Sun Woo Jin, Gi Ho Lee & Hye Gwang Jeong

  2. Department of Horticulture, Chunnam Techno University, Gokseong, 57500, Republic of Korea

    Song Mi Cho

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  1. Jae Ho Choi
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  2. Sun Woo Jin
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  3. Gi Ho Lee
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Correspondence to Song Mi Cho or Hye Gwang Jeong.

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Choi, J.H., Jin, S.W., Lee, G.H. et al. Orostachys japonicus ethanol extract inhibits 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice and TNF-α/IFN-γ-induced TARC expression in HaCaT cells. Toxicol Res. 36, 99–108 (2020). https://doi.org/10.1007/s43188-019-00026-0

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