Skip to main content
Log in

Direct recovery of intracellular lipase from cell lysate by adsorption on silica magnetic microparticles activated with Octyl groups

Brazilian Journal of Chemical Engineering Aims and scope Submit manuscript

Abstract

New efficient protein purification methods need to be developed to overcome product loss during purification and to lower its final price. Magnetic adsorbents emerge as a potential support which can easily separate target biomolecules from crude biological medium. The hydrophobic adsorption of recombinant lipase from Bacillus thermocatenulatus (BTL-2) in novel silica magnetic microparticles (SMMp) was investigated aiming to directly recover it from cell lysate. The adsorbent surface of SMMp was modified by activation with octyl groups (25%–100%). Adsorptions on silica-octyl (non-magnetic) were also performed to understand the process better. Using centrifuged and dialyzed enzyme, under low ionic strength, the highest enzyme adsorption (92%) and desorption yields (50%) were obtained using SMMp-octyl-75% and SMMp-octyl-25%, respectively, due to simultaneous hydrophobic and ionic interaction between charged silanol groups and the enzyme. Direct enzyme adsorption from cell lysates under high ionic strength conditions led to a high yield of enzyme adsorption with 100% desorption using SMMp-octyl-25%. Therefore, the use of SMMp-octyl allows for a simple and highly efficient BTL-2 recovery by a one-step purification technique that can even be extended to other applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Data availability

All data presented in this article, including Supplementary Material, are available from the corresponding author on reasonable request.

Code availability

Not applicable.

References

Download references

Acknowledgements

The authors are grateful to the Laboratory of Biocatalysis (ICP-CSIC, Madrid, Spain) for the donation of recombinant cells of E. coli, and the São Paulo Research Foundation (FAPESP grants #2008/56246-0 and 2016/10636-8), the Brazilian National Council for Scientific and Technological Development (CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support.

Funding

This work was supported by the São Paulo Research Foundation (FAPESP), grants #2008/56246-0 and 2016/10636-8, and the Brazilian National Council for Scientific and Technological Development (CNPq) for their financial support. This work was in part financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (CAPES) – Finance code 001.

Author information

Authors and Affiliations

Authors

Contributions

All authors contributed to the study conception and design. LAL performed all experiments with the support of WK. All authors discussed and analysed the data. The first draft of the manuscript was written by LAL and manuscript review and editing were carried out by TSM and TP. RLCG revised the manuscript and supervised the work development. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Raquel L. C. Giordano.

Ethics declarations

Conflicts of interest

The authors declare that they have no conflicts and competing interests.

Ethics approval

Not applicable.

Consent to participate

Not applicable.

Consent for publication

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 114 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lopes, L.A., Kopp, W., Milessi, T.S. et al. Direct recovery of intracellular lipase from cell lysate by adsorption on silica magnetic microparticles activated with Octyl groups. Braz. J. Chem. Eng. 39, 789–801 (2022). https://doi.org/10.1007/s43153-021-00190-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s43153-021-00190-7

Keywords

Navigation