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Stability studies of β-galactosidase immobilized on gluconic acid coated fullerenes

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Abstract

The present study demonstrates the coating of fullerenes with gluconic acid for the immobilization of Aspergillus oryzae β-galactosidase. The prepared nanomatrix provided 86% immobilization yield, and broadened the biocatalytic activity of immobilized enzyme at higher pH and temperature ranges. Immobilized β-galactosidase exhibited 60% activity even at 5.0% galactose concentration as compared to 23% enzyme activity obtained by soluble enzyme under similar experimental conditions. Reusability of the enzyme was improved considerably as a result of covalent immobilization. Immobilized β-galactosidase showed 89% activity even after sixth repeated use and could be recovered easily. The noticeable improvement in lactose hydrolysis was observed in batch reactors by immobilized enzyme in contrast to the soluble enzyme at high temperature ranges. At 50 °C, 89% lactose conversion was achieved by immobilized enzyme as compared to 77%, obtained by free β-galactosidase under similar incubation conditions. Hence, the developed immobilized enzyme preparation could be exploited for converting lactose into its monosaccharides in a convenient and cheaper way.

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Abbreviations

GACF:

Gluconic acid coated fullerene

IβG:

Immobilized β-galactosidase (β-galactosidase bound to GACF)

NPs:

Nanoparticles

SβG:

Soluble β-galactosidase

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Acknowledgements

Mr. Jitendra Kumar of Department of Biotechnology (Chaudhary Charan Singh University, India) is gratefully acknowledged for providing the fullerene used in the study.

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Batterjee Medical College for Sciences and Technology, Jeddah, Saudi Arabia

    Shakeel Ahmed Ansari

  2. Department of Community Medicine and Pilgrims Health Care, Umm Alqura University, Makkah, Saudi Arabia

    Asim Muhammed Alshanberi

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  1. Shakeel Ahmed Ansari
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  2. Asim Muhammed Alshanberi
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Correspondence to Shakeel Ahmed Ansari.

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Ansari, S.A., Alshanberi, A.M. Stability studies of β-galactosidase immobilized on gluconic acid coated fullerenes. Braz. J. Chem. Eng. 39, 361–367 (2022). https://doi.org/10.1007/s43153-021-00146-x

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  • DOI: https://doi.org/10.1007/s43153-021-00146-x

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