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Tannic acid alleviates 3-nitropropionic acid-induced ovarian damage in Brandt’s vole (Lasiopodomys brandtii)

  • Reproductive Endocrinology: Original Article
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Abstract

Tannic acid (TA) is a polyphenol with antioxidant properties present in various plants. In this study, we explored the protective effect of TA against ovarian oxidative stress in Brandt’s voles and its underlying mechanism. At various doses, 3-nitropropionic acid (3-NPA) was intraperitoneally injected into Brandt’s voles to simulate ovarian oxidative stress. Thereafter, various doses of TA were intragastrically administered to examine the protective effect of TA against 3-NPA-induced ovarian damage. Changes in inflammation, autophagy, apoptosis, and oxidative stress-related factors were investigated through various biochemical and histological techniques. Ovarian oxidative stress was successfully induced by the intraperitoneal administration of 12.5 mg/kg 3-NPA for 18 days. As a result, the ovarian coefficient decreased and ovarian tissue fibrosis was induced. TA treatment effectively alleviated the increase in luteinizing hormone and follicle-stimulating hormone levels; the decrease in estradiol, progesterone, and anti-Müllerian hormone levels; and the decline in fertility induced by 3-NPA. Compared to that in the 3-NPA group, TA decreased the expression of autophagy-related proteins beclin-1 and LC3, as well as the level of apoptosis. It also activated the AKT/mTOR signaling pathway, downregulated PTEN and p-NF-κB expression, and upregulated Nrf2 expression. In conclusion, our findings indicate that TA could inhibit autophagy via the regulation of AKT/mTOR signaling, suppressing oxidative damage and inflammatory responses through Nrf2 to alleviate 3-NPA-induced ovarian damage. Collectively, the current findings highlight the protective effects of TA in Brandt’s vole, where it promotes the maintenance of normal ovarian function.

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Funding

This work was supported by the National Natural Science Foundation of China [grant number: 31971418] and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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  1. Minghao Yu and Sheng-Mei Yang contributed equally to this work.

Authors and Affiliations

  1. School of Food and Biological Engineering, Yantai Institute of Technology, Yantai, 264003, China

    Minghao Yu

  2. Department of College of Biological Science and Technology, Yangzhou University, Yangzhou, 225009, China

    Minghao Yu, Ruiyang Fan, Daochen Wang, Yuxuan Han, Xin Dai & Sheng-Mei Yang

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  1. Minghao Yu
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Contributions

Ming-Hao Yu designed and performed the experiments and analyzed the data; Rui-Yang Fan, Dao-Chen Wang and Yu-Xuan Han designed the experiments and collected the data. Xin Dai and Sheng-Mei Yang revised and edited the manuscript. All authors read and accepted the manuscript.

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Correspondence to Minghao Yu or Sheng-Mei Yang.

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Yu, M., Fan, R., Wang, D. et al. Tannic acid alleviates 3-nitropropionic acid-induced ovarian damage in Brandt’s vole (Lasiopodomys brandtii). Reprod. Sci. (2024). https://doi.org/10.1007/s43032-024-01543-6

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